Biomimetic microcavities based on poly(dimethylsiloxane) elastomers
Biomimetic microcavities based on poly(dimethylsiloxane) elastomers
复制标题
基于聚二甲基硅氧烷弹性体的仿生微腔
作者：
Katja Franke;I. Kurth;M. Bornhäuser;C. Werner;T. Pompe
我们报告了一种新的技术，用于制备仿生细胞培养载体的基础上聚（二甲基硅氧烷）（PDMS），以提供精确定义的微腔结合共价连接的功能生物分子的选择。通过共价连接的聚（乙烯-alt-马来酸酐）层对直径为10-80 µm且深度为10 µm的微腔尺寸的PDMS模具进行修饰，以允许共价或物理吸附连接从细胞外基质蛋白到生长因子到糖胺聚糖的生物分子。此外，聚（乙烯-交替-马来酸酐）层为小的有机硅组分可能扩散出PDMS块体基底提供了有效的屏蔽。我们采用固定化技术将细胞外基质成分纤连蛋白、层粘连蛋白、肝素和透明质酸固定在规定的表面量，其中纤连蛋白配体为1.4 × 1012/cm 2，层粘连蛋白配体为5.2 × 1011/cm 2，肝素二糖单位为5.6 × 1014/cm 2，透明质酸二糖单位为6 × 1014/cm 2。仿生微腔作为多功能和稳健的支架用于控制和研究细胞命运决定的功能利用被证明用于造血干细胞（HSC）。微腔尺寸的影响表现在与40 μm直径的微腔相比，15 μm直径的肝素包被的微腔上的细胞增殖活性降低。这种行为是叠加的纤维连接蛋白肝素涂层上的HSC增殖的额外减少。稳定的HSC表面标志物表达（CD 34和CD 133）的增殖抑制小微腔和高活力的细胞表明，由于增强的接触HSC与细胞外基质的更静止的状态。
We report on a new technology for preparing biomimetic cell culture carriers based on poly(dimethylsiloxane) (PDMS) to provide precisely defined microcavities combined with an option for covalent attachment of functional biomolecules. PDMS molds with a microcavity size of 10–80 µm in diameter and a depth of 10 µm were modified by a covalently attached poly(ethene-alt-maleic anhydride) layer to allow covalent or physisorptive attachment of biomolecules ranging from extracellular matrix proteins through growth factors to glycosaminoglycans. The poly(ethene-alt-maleic anhydride) layer furthermore provided an effective shielding for the possible diffusion of small silicone components out of the PDMS bulk substrate. We employ the immobilization technology to the extracellular matrix components fibronectin, laminin, heparin, and hyaluronic acid in defined surface amounts with 1.4 × 1012 fibronectin ligands per cm2, 5.2 × 1011laminin ligands per cm2, 5.6 × 1014heparin disaccharide units per cm2, and 6 × 1014hyaluronic acid disaccharide units per cm2. The functional utilization of the biomimetic microcavities as versatile and robust scaffolds for controlling and investigating cell fate decisions was demonstrated for hematopoietic stem cells (HSC). The effect of microcavity size shows up in decreased proliferative activity of the cells on heparin-coated microcavities of 15 µm diameter in comparison to 40 µm ones. This behavior is superimposed by an additional decrease in proliferation of HSC on fibronectin versusheparin coatings. A stable HSC surface marker expression (CD34 and CD133) on proliferation suppressing small microcavities and the high viability of the cells suggested a more quiescent state due to an enhanced contact of HSC with the presented extracellular matrix.
影响因子：
56.9
作者：
Chen, CS;Mrksich, M;Ingber, DE
通讯作者：
Ingber, DE
影响因子：
15.9
作者：
HURLEY, RW;MCCARTHY, JB;VERFAILLIE, CM
通讯作者：
VERFAILLIE, CM
影响因子：
48
作者：
Alberti, Kristin;Davey, Ryan E.;Zandstra, Peter W.
通讯作者：
Zandstra, Peter W.