Bilateral symmetry of linear streptomycete chromosomes.
Bilateral symmetry of linear streptomycete chromosomes.
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DOI：
10.1099/mgen.0.000692
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发表时间：
2021-11
影响因子：
3.9
通讯作者：
Herron PR
中科院分区：
文献类型：
--
作者：
Algora-Gallardo L;Schniete JK;Mark DR;Hunter IS;Herron PR
在这里，我们描述了一组不常见的端粒，从链霉菌atcc 10970，父母菌株的一个谱系的最早发现的抗生素生产商之一。在其基因组序列关闭后，我们将这种有机体中不寻常的端粒与链霉菌中发现的其他五类复制子末端进行了比较。根据系统发育和物理定位，链霉菌染色体的闭合复制子被组织起来，这表明不同的端粒与特定的分支无关，可能是通过质粒驱动的水平基因转移在不同菌株之间共享的。此外，我们还发现了一个~50kb的同步性保守的起源岛，它位于所有链霉菌染色体的核心，形成了一个可以发生对称性染色体倒置的轴。尽管存在这种染色体的双边对称性，但在链霉菌科中，ORIC右侧的PARS位点仍然存在偏向，这表明在链霉菌的右侧复制核上形成PARB/PARS核蛋白复合体是链霉菌的一个保守特征。因此，我们的研究揭示了线性细菌复制子的新特征，通过对它们的操纵，可能会提高这一重要的工业细菌群体的生长和生产率。
Here, we characterize an uncommon set of telomeres from Streptomyces rimosus ATCC 10970, the parental strain of a lineage of one of the earliest-discovered antibiotic producers. Following the closure of its genome sequence, we compared unusual telomeres from this organism with the other five classes of replicon ends found amongst streptomycetes. Closed replicons of streptomycete chromosomes were organized with respect to their phylogeny and physical orientation, which demonstrated that different telomeres were not associated with particular clades and are likely shared amongst different strains by plasmid-driven horizontal gene transfer. Furthermore, we identified a ~50 kb origin island with conserved synteny that is located at the core of all streptomycete chromosomes and forms an axis around which symmetrical chromosome inversions can take place. Despite this chromosomal bilateral symmetry, a bias in parS sites to the right of oriC is maintained across the family Streptomycetaceae and suggests that the formation of ParB/parS nucleoprotein complexes on the right replichore is a conserved feature in streptomycetes. Consequently, our studies reveal novel features of linear bacterial replicons that, through their manipulation, may lead to improvements in growth and productivity of this important industrial group of bacteria.
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影响因子：
3.6
作者：
Huang, CH;Chen, CY;Chen, CW
通讯作者：
Chen, CW
影响因子：
11.4
作者：
EVANS, M;DYSON, P
通讯作者：
DYSON, P
影响因子：
14.9
作者：
Alanjary, Mohammad;Steinke, Katharina;Ziemert, Nadine
通讯作者：
Ziemert, Nadine
DOI：
10.1099/ijs.0.058107-0
发表时间：
2014-03-01
影响因子：
2.8
作者：
Labeda, D. P.;Doroghazi, J. R.;Metcalf, W. W.
通讯作者：
Metcalf, W. W.
影响因子：
3.4
作者：
Gomez-Escribano JP;Holmes NA;Schlimpert S;Bibb MJ;Chandra G;Wilkinson B;Buttner MJ;Bibb MJ
通讯作者：
Bibb MJ