Functional characterization and axonal transport of quantum dot labeled BDNF.
Functional characterization and axonal transport of quantum dot labeled BDNF.
复制标题
DOI：
10.1039/c2ib20062g
复制
发表时间：
2012-08
期刊：
影响因子：
--
通讯作者：
Cui B
中科院分区：
文献类型：
--
作者：
Xie W;Zhang K;Cui B
脑源性神经营养因子（BDNF）在中枢和外周神经系统的生长、发育和维持中起着关键作用。外源性BDNF激活轴突终末的膜受体，随后向细胞体发送调节信号。为了了解BDNF信号如何在神经元中传播，重要的是要跟踪BDNF在轴突末端内化后的运输。本研究用明亮的光稳定量子点（QD-BDNF）标记BDNF，并在海马神经元中真实的实时追踪了QD-BDNF的轴突转运。我们发现QD-BDNF能够结合BDNF受体并激活下游信号通路。当QD-BDNF作用于海马神经元的远端轴突时，观察到其以1.11 ± 0.05 μm/s的平均速度被主动运输到细胞体。更仔细的检查显示，QD-BDNF被离散的内体和多泡体样结构转运。我们的研究结果表明，QD-BDNF可用于长距离追踪外源性BDNF在神经元中的运动，并研究含有BDNF的信号细胞器。
Brain derived neurotrophic factor (BDNF) plays a key role in the growth, development and maintenance of the central and peripheral nervous systems. Exogenous BDNF activates its membrane receptors at the axon terminal, and subsequently sends regulation signals to the cell body. To understand how BDNF signal propagates in neurons, it is important to follow the trafficking of BDNF after it is internalized at the axon terminal. Here we labeled BDNF with bright, photostable quantum dot (QD-BDNF) and followed the axonal transport of QD-BDNF in real time in hippocampal neurons. We showed that QD-BDNF was able to bind BDNF receptors and activate downstream signaling pathways. When QD-BDNF was applied to the distal axons of hippocampal neurons, it was observed to be actively transported toward the cell body at an average speed of 1.11 ± 0.05 μm/s. A closer examination revealed that QD-BDNF was transported by both discrete endosomes and multivesicular body-like structures. Our results showed that QD-BDNF could be used to track the movement of exogenous BDNF in neurons over long distances and to study the signaling organelles that contain BDNF.
登录
查看更多内容
DOI：
10.1002/cne.22047
发表时间：
2009-06-20
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
Altick AL;Baryshnikova LM;Vu TQ;von Bartheld CS
通讯作者：
von Bartheld CS
DOI：
10.1002/neu.10323
发表时间：
2004-02-05
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Howe, CL;Mobley, WC
通讯作者：
Mobley, WC
影响因子：
16.2
作者：
ALDERSON, RF;ALTERMAN, AL;LINDSAY, RM
通讯作者：
LINDSAY, RM
影响因子：
2.4
作者：
Adachi, N;Kohara, K;Tsumoto, T
通讯作者：
Tsumoto, T
DOI：
10.1073/pnas.0706192104
发表时间：
2007-08-21
影响因子：
11.1
作者：
Cui, Bianxiao;Wu, Chengbiao;Chu, Steven
通讯作者：
Chu, Steven