Comparison of sputum microbiome of legionellosis-associated patients and other pneumonia patients: indications for polybacterial infections.
Comparison of sputum microbiome of legionellosis-associated patients and other pneumonia patients: indications for polybacterial infections.
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DOI：
10.1038/srep40114
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发表时间：
2017-01-06
影响因子：
4.6
通讯作者：
Halpern M
中科院分区：
文献类型：
--
作者：
Mizrahi H;Peretz A;Lesnik R;Aizenberg-Gershtein Y;Rodríguez-Martínez S;Sharaby Y;Pastukh N;Brettar I;Höfle MG;Halpern M
军团菌属的细菌引起水基感染，导致严重的肺炎。在这里，我们分析和比较了肺炎患者痰液样本的细菌微生物组与军团菌属的存在和丰度的关系。通过培养、PCR和下一代测序（NGS）确定军团菌属的患病率。使用军团菌属特异性引物分析的133份痰液样本中有9份为PCR阳性。只有一个样本是阳性的文化。对军团菌阳性和军团菌阴性痰液样本的Illumina MiSeq 16S rRNA基因测序分析证实，军团菌确实存在于PCR阳性痰液样本中。这种方法允许在属水平上鉴定痰微生物组，并在种和亚种水平上鉴定军团菌属。42%的痰标本以链球菌为主。军团菌从来不是主要的属，总是伴随着其他呼吸道病原体。有趣的是，军团菌阳性的痰液样本被观察到与阿米巴相关的水生细菌所占据，这表明阿米巴可能将军团菌与其微生物组一起从饮用水中转移。这是第一项研究，证明痰液中主要细菌和病原体的档案军团菌的存在。
Bacteria of the genus Legionella cause water-based infections resulting in severe pneumonia. Here we analyze and compare the bacterial microbiome of sputum samples from pneumonia patients in relation to the presence and abundance of the genus Legionella. The prevalence of Legionella species was determined by culture, PCR, and Next Generation Sequencing (NGS). Nine sputum samples out of the 133 analyzed were PCR-positive using Legionella genus-specific primers. Only one sample was positive by culture. Illumina MiSeq 16S rRNA gene sequencing analyses of Legionella-positive and Legionella-negative sputum samples, confirmed that indeed, Legionella was present in the PCR-positive sputum samples. This approach allowed the identification of the sputum microbiome at the genus level, and for Legionella genus at the species and sub-species level. 42% of the sputum samples were dominated by Streptococcus. Legionella was never the dominating genus and was always accompanied by other respiratory pathogens. Interestingly, sputum samples that were Legionella positive were inhabited by aquatic bacteria that have been observed in an association with amoeba, indicating that amoeba might have transferred Legionella from the drinking water together with its microbiome. This is the first study that demonstrates the sputum major bacterial commensals and pathogens profiles with regard to Legionella presence.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
9.8
作者：
Blanky, Marina;Rodriguez-Martinez, Sara;Friedler, Eran
通讯作者：
Friedler, Eran
影响因子：
11.8
作者：
Garrido, RMB;Parra, FJE;Huerta, FH
通讯作者：
Huerta, FH
影响因子：
4.4
作者：
Kahlisch, Leila;Henne, Karsten;Hoefle, Manfred G.
通讯作者：
Hoefle, Manfred G.
影响因子：
28.2
作者：
Gadsby, Naomi J.;Helgason, Kristjan O.;Templeton, Kate E.
通讯作者：
Templeton, Kate E.