Differential localization of colonic H(+)-K(+)-ATPase isoforms in surface and crypt cells.
Differential localization of colonic H(+)-K(+)-ATPase isoforms in surface and crypt cells.
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结肠 H( )-K( )-ATP 酶亚型在表面和隐窝细胞中的差异定位。
DOI：
10.1152/ajpgi.1998.274.2.g424
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Binder,HJ
中科院分区：
文献类型：
--
作者：
Rajendran,VM;Singh,SK;Geibel,J;Binder,HJ
两种不同的结肠H+-K+-腺苷三磷酸酶（H+-K+- atp酶）异构体可以部分地根据它们对瓦巴因的敏感性来识别。大肠H+-K+- atp酶α-亚基（HKcα）最近被克隆出来，其信息和蛋白存在于大鼠远端结肠表面（和隐窝上部20%）细胞中。这些研究是为了确定由表面和隐窝细胞制备的根尖膜中H+-K+- atp酶活性的瓦阿巴因敏感成分和瓦阿巴因不敏感成分的空间分布，以及分离的灌注结肠隐窝和表面上皮细胞中酸负荷后细胞内K+依赖性pH （pHi）的恢复。表面细胞根尖膜上的H+-K+- atp酶活性对瓦阿因敏感46%，而隐窝根尖膜上的H+-K+- atp酶活性对瓦阿因敏感96%。同样，在离体隐窝中，K+依赖的pHirecovery对瓦巴因完全敏感，而在表面细胞中，K+依赖的pHirecovery对瓦巴因不敏感。这些研究提供了令人信服的证据，证明HKcα编码结肠对瓦塔因不敏感的H+-K+- atp酶，并且结肠对瓦塔因敏感的H+-K+- atp酶异构体存在于结肠隐窝中，仍有待克隆和鉴定。
Two distinct colonic H+-K+-adenosinetriphosphatase (H+-K+-ATPase) isoforms can be identified in part on the basis of their sensitivity to ouabain. The colonic H+-K+-ATPase α-subunit (HKcα) was recently cloned, and its message and protein are present in surface (and the upper 20% of crypt) cells in the rat distal colon. These studies were performed to establish the spatial distribution of the ouabain-sensitive and ouabain-insensitive components of both H+-K+-ATPase activity in apical membranes prepared from surface and crypt cells and K+-dependent intracellular pH (pHi) recovery from an acid load both in isolated perfused colonic crypts and in surface epithelial cells. Whereas H+-K+-ATPase activity in apical membranes from surface cells was 46% ouabain sensitive, its activity in crypt apical membranes was 96% ouabain sensitive. Similarly, K+-dependent pHirecovery in isolated crypts was completely ouabain sensitive, whereas in surface cells K+-dependent pHirecovery was insensitive to ouabain. These studies provide compelling evidence that HKcα encodes the colonic ouabain-insensitive H+-K+-ATPase and that a colonic ouabain-sensitive H+-K+-ATPase isoform is present in colonic crypts and remains to be cloned and identified.
影响因子：
4.7
作者：
LUNN, G;SANSONE, EB;KEEFER, LK
通讯作者：
KEEFER, LK
诱变剂对带有共价键三磺基铜酞菁的棉花的吸附。
DOI：
--
发表时间：
1983
期刊：
Mutation research
影响因子：
--
作者：
H. Hayatsu;T. Oka;A. Wakata;Y. Ohara;T. Hayatsu;H. Kobayashi;S. Arimoto
通讯作者：
S. Arimoto
一些多核烃的致癌性和致突变性之间的关系。
DOI：
10.1016/0027-5107(78)90120-3
发表时间：
1978
期刊：
Mutation research
影响因子：
--
作者：
A. W. Andrews;L. Thibault;W. Lijinsky
通讯作者：
W. Lijinsky