PTS1 peroxisomal import pathway plays shared and distinct roles to PTS2 pathway in development and pathogenicity of Magnaporthe oryzae.
PTS1 peroxisomal import pathway plays shared and distinct roles to PTS2 pathway in development and pathogenicity of Magnaporthe oryzae.
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PTS1 过氧化物酶体输入途径与 PTS2 途径在稻瘟病菌的发育和致病性中发挥共同和独特的作用
DOI：
10.1371/journal.pone.0055554
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Sun G
中科院分区：
文献类型：
--
作者：
Wang J;Zhang Z;Wang Y;Li L;Chai R;Mao X;Jiang H;Qiu H;Du X;Lin F;Sun G
过氧化物酶体参与各种重要的代谢，是真菌植物病原体致病性所必需的。过氧化物酶体基质蛋白通过过氧化物酶体靶向信号 1 (PTS1) 或过氧化物酶体靶向信号 2 (PTS2) 输入途径从细胞质输入过氧化物酶体。 PEX5和PEX7基因分别参与这两条途径。 PEX7 介导的 PTS2 输入途径参与真菌致病性已被证实，而 PTS1 的参与尚不清楚。通过对稻瘟病菌中 PEX5 同源物 MoPEX5 的无效突变分析，我们报告了 PTS1 途径在稻瘟病菌发育和宿主感染中的关键作用，并与 PTS2 进行了比较。我们发现 MoPEX5 破坏特异性阻断了 PTS1 通路。 Δmopex5无法利用脂质作为唯一的碳源并完全丧失致病性。与 Δmopex7 类似，Δmopex5 在脂质利用和动员、附着膨压发生和 H2O2 抗性方面表现出显着降低。此外，Δmopex5 在营养生长、分生孢子形态发生、附着形态发生和黑化方面表现出一些在 Δmopex7 中未检测到的明显缺陷。结果表明，除PTS2外，PTS1过氧化物酶体输入途径也是稻瘟菌真菌发育和致病性所必需的，并且作为主要的过氧化物酶体输入途径，比PTS2发挥更重要的作用。
Peroxisomes participate in various important metabolisms and are required in pathogenicity of fungal plant pathogens. Peroxisomal matrix proteins are imported from cytoplasm into peroxisomes through peroxisomal targeting signal 1 (PTS1) or peroxisomal targeting signal 2 (PTS2) import pathway. PEX5 and PEX7 genes participate in the two pathways respectively. The involvement of PEX7 mediated PTS2 import pathway in fungal pathogenicity has been documented, while that of PTS1 remains unclear. Through null mutant analysis of MoPEX5, the PEX5 homolog in Magnaporthe oryzae, we report the crucial roles of PTS1 pathway in the development and host infection in the rice blast fungus, and compared with those of PTS2. We found that MoPEX5 disruption specifically blocked the PTS1 pathway. Δmopex5 was unable to use lipids as sole carbon source and lost pathogenicity completely. Similar as Δmopex7, Δmopex5 exhibited significant reduction in lipid utilization and mobilization, appressorial turgor genesis and H2O2 resistance. Additionally, Δmopex5 presented some distinct defects which were undetected in Δmopex7 in vegetative growth, conidial morphogenesis, appressorial morphogenesis and melanization. The results indicated that the PTS1 peroxisomal import pathway, in addition to PTS2, is required for fungal development and pathogenicity of the rice blast fungus, and also, as a main peroxisomal import pathway, played a more predominant role than PTS2.
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影响因子：
2.9
作者：
Amano, T;Hirasawa, K;Shioi, Y
通讯作者：
Shioi, Y
影响因子：
4.8
作者：
Hayashi, M;Yagi, M;Nishimura, M
通讯作者：
Nishimura, M
DOI：
10.1007/bf00282757
发表时间：
1994-09-28
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
作者：
COE, JGS;MURRAY, LE;DAWES, IW
通讯作者：
DAWES, IW
影响因子：
7.7
作者：
Einwächter, H;Sowinski, S;Schliebs, W
通讯作者：
Schliebs, W
影响因子：
3.5
作者：
Braverman, N;Dodt, G;Valle, D
通讯作者：
Valle, D