Characterization of the polarized endothelial secretome
Characterization of the polarized endothelial secretome
复制标题
极化内皮分泌组的表征
DOI：
--
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
H. Mellor
中科院分区：
文献类型：
--
作者：
Haoche Wei;Ananthalakshmy Sundararaman;E. Dickson;Lewis Rennie;Eloise Cross;K. Heesom;H. Mellor
血管内皮细胞(ECs)在循环和身体之间形成了一道活跃的屏障。除了控制这两个隔室之间的分子运输外，内皮还是一个主要的分泌器官，将蛋白质释放到循环和血管基质中。尽管蛋白质被正确地分选到这两个空间显然很重要，但我们目前对这种分泌的极化或它是如何控制的知之甚少。在这里，我们提出了一个分析极化分泌物的优化系统，并表明它允许从少量的原代内皮细胞中衍生出深层的、强大的蛋白质组。我们提出了第一个内皮细胞顶端和基底侧面分泌的蛋白质组，表明内皮细胞极化了细胞外小泡货物到根尖表面的分泌。相反，我们发现基底外侧表面的蛋白质分泌集中在细胞外基质(ECM)的成分上。最后，我们研究了Liprin-α1在向基底外侧室分泌过程中的作用，并确定了与纤维连接蛋白共享这一途径的细胞外基质成分的子集。FASE B J.33,12277-12287(2019年)。Www.fasebj.org
Endothelial cells (ECs) form an active barrier between the circulation and the body. In addition to controlling transport of molecules between these 2 compartments, the endothelium is a major secretory organ, releasing proteins both into the circulation and into the vascular matrix. Although it is clearly important that proteins are correctly sorted into these 2 spaces, we currently know little of the polarization of this secretion or how it is controlled. Here, we present an optimized system for the analysis of polarized secretion and show that it allows the derivation of deep, robust proteomes from small numbers of primary ECs. We present the first endothelial apically and basolaterally secreted proteomes, demonstrating that ECs polarize the secretion of extracellular vesicle cargoes to the apical surface. Conversely, we find that protein secretion at the basolateral surface is focused on components of the extracellular matrix (ECM). Finally, we examine the role of liprin‐α1 in secretion toward the basolateral compartment and identify a subset of ECM components that share this route with fibronectin.—Wei, H., Sundararaman, A., Dickson, E., Rennie‐Campbell, L., Cross, E., Heesom, K. J., Mellor, H. Characterization of the polarized endothelial secretome. FASEB J. 33, 12277‐12287 (2019). www.fasebj.org
血浆磷脂转移蛋白在脂质和脂蛋白代谢中的作用。
DOI：
10.1016/j.bbalip.2011.06.013
发表时间：
2012-03
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Albers JJ;Vuletic S;Cheung MC
通讯作者：
Cheung MC
影响因子：
82.9
作者：
Korf-Klingebiel, Mortimer;Reboll, Marc R.;Wollert, Kai C.
通讯作者：
Wollert, Kai C.
极化纤连蛋白分泌和局部基质组装位点与内皮下基质形成相关。
影响因子：
20.3
作者：
Kowalczyk,AP;Tulloh,RH;McKeown-Longo,PJ
通讯作者：
McKeown-Longo,PJ