Aberrant FHIT transcripts in cancerous and corresponding non‐cancerous lesions of the digestive tract
Aberrant FHIT transcripts in cancerous and corresponding non‐cancerous lesions of the digestive tract
复制标题
消化道癌性病变和相应非癌性病变中的异常 FHIT 转录本
作者：
Yi;P. Chen;Meng‐Dar Lee;Jan
FHIT基因最近被发现并被提议为肿瘤抑制基因。我们通过逆转录FHIT mRNA，随后进行PCR扩增和产物测序，检测了一组消化道癌沿着及其相应非肿瘤组织中的FHIT基因。发现正常FHIT转录物在46个癌组织中的44个和测试的所有46个非癌组织中以稳健水平表达。在46例癌组织中，21例（45.7%）发现异常转录本的发生。另外，在46例匹配的正常组织中，14例（30.4%）显示存在异常转录本。序列分析证实FHIT cDNA的一个或多个外显子中缺失异常转录本，而8例病例在癌组织和非癌组织中均显示FHIT基因的异常转录本。然而，序列分析显示，相应的配对样品之间的异常转录本的模式是不同的。此外，有6例仅在其正常组织对应物中显示异常FHIT转录物。这些研究表明，FHIT基因转录物的异常在消化道的癌性和相应的非癌性病变中以相当高的频率发生。然而，它们可能与消化道肿瘤的发生没有因果关系。Int. J. Cancer 72：955-958，1997.© 1997 Wiley利斯公司
The FHIT gene was recently discovered and proposed as a tumor‐suppressor gene. We examined the FHIT gene in a panel of digestive‐tract cancers along with their corresponding non‐tumorous tissues by reverse transcription of FHIT mRNA followed by PCR amplification and sequencing of the products. A normal FHIT transcript was found to be expressed at robust levels in 44 of 46 cancerous tissues and in all of 46 non‐cancerous tissues tested. Of the 46 cancerous specimens, 21 (45.7%) revealed the occurrence of aberrant transcripts. In addition, of the 46 matched normal tissues, 14 (30.4%) showed the existence of aberrant transcripts. Sequence analysis confirmed that aberrant transcripts were missing in one or more exons of the FHIT cDNA, while 8 cases displayed aberrant transcripts of the FHIT gene both in cancerous and in non‐cancerous tissues. However, sequence analysis revealed that the patterns of the aberrant transcripts were different between the corresponding paired samples. In addition, there were 6 cases displaying aberrant FHIT transcripts only in their normal‐tissue counterparts. These studies indicate that abnormalities of the FHIT gene transcripts occur at a fairly high frequency in cancerous and corresponding non‐cancerous lesions of the digestive tract. However, they might not be causally related to the tumorigenesis of the digestive tract. Int. J. Cancer 72:955–958, 1997. © 1997 Wiley‐Liss, Inc.
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影响因子：
4.4
作者：
W. Paradee;C. Mullins;Z. He;T. Glover;C. Wilke;B. Opalka;J. Schutte;D. Smith
通讯作者：
W. Paradee;C. Mullins;Z. He;T. Glover;C. Wilke;B. Opalka;J. Schutte;D. Smith
影响因子：
2.9
作者：
Barnes, LD;Garrison, PN;Huebner, F
通讯作者：
Huebner, F
通过肿瘤细胞系中的纯合缺失鉴定出 3p14.2 FRA3B 位点的潜在胃肠道肿瘤抑制基因座。
影响因子：
11.2
作者：
Kastury,K;Baffa,R;Druck,T;Ohta,M;Cotticelli,MG;Inoue,H;Negrini,M;Rugge,M;Huang,D;Croce,CM;Palazzo,J;Huebner,K
通讯作者：
Huebner,K
影响因子：
11.2
作者：
T. Druck;P. Hadaczek;Tie-bo Fu;M. Ohta;Z. Siprashvili;R. Baffa;M. Negrini;K. Kastury;M. L. Veronese;D. Rosen;J. Rothstein;P. Mccue;M. Cotticelli;H. Inoue;C. Croce;K. Huebner
通讯作者：
T. Druck;P. Hadaczek;Tie-bo Fu;M. Ohta;Z. Siprashvili;R. Baffa;M. Negrini;K. Kastury;M. L. Veronese;D. Rosen;J. Rothstein;P. Mccue;M. Cotticelli;H. Inoue;C. Croce;K. Huebner
结直肠癌中 FHIT 基因的评估。
影响因子：
11.2
作者：
Thiagalingam,S;Lisitsyn,NA;Hamaguchi,M;Wigler,MH;Willson,JK;Markowitz,SD;Leach,FS;Kinzler,KW;Vogelstein,B
通讯作者：
Vogelstein,B