Resistance Sources to Brown Blotch Disease (Pseudomonas tolaasii) in a Diverse Collection of Pleurotus Mushroom Strains

Resistance Sources to Brown Blotch Disease (Pseudomonas tolaasii) in a Diverse Collection of Pleurotus Mushroom Strains
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不同的侧耳蘑菇菌株对褐斑病(托拉假单胞菌)的抗性来源

DOI:
10.3390/pathogens8040227
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发表时间:
2019-11
期刊:
影响因子:
3.7
通讯作者:
Yu Li
Yu Li
中科院分区:
医学3区
文献类型:
--
作者:
Benjamin Azu Okorley;Frederick Leo Sossah;Dan Dai;Shuai Xu;Zhenghui Liu;Bing Song;Hongyan Sheng;Yongping Fu;Yu Li

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由假单胞菌引起的褐斑病是侧耳属植物的主要病害之一。国际吧选育抗病菌株是防治BBD的最有效方法。为了筛选出对BBD具有抗性的种质资源,对97个菌株(包括21个P.cf. floridanus、20株P.ostreatus和56株P.pulmonarius通过两种不同的方法进行筛选;即,在受控条件下将病原体接种在蘑菇菌盖(IMP)和产卵基质(IMSS)上。在97个菌株中,22个为肺原杆菌,4个为cf.使用IMP方法,floridanus对BBD具有中度抗性。11个肺原杆菌,6个肺原杆菌。florida和1个糙皮侧耳菌株对BBD具有高抗性。所有的97株显示不同程度的敏感性使用IMP方法,但8株完全耐药使用IMSS方法。结合这两种方法,五个菌株是高度耐药的(四个肺原杆菌和一个肺原杆菌)。floridanus)和11个中度耐药(8个肺原蜱和3个P. cf. floridanus)。肺吸虫和肺吸虫对托氏肺吸虫的耐药来源。floridanus可用于侧耳属的进一步育种。
Brown blotch disease (BBD) caused by Pseudomonas tolaasii is one of the most devastating diseases of Pleurotus spp. worldwide. Breeding for resistant strains is the most effective method for controlling BBD. To identify resistant germplasm for BBD management, 97 strains comprising 21 P. cf. floridanus, 20 P. ostreatus, and 56 P. pulmonarius were screened by two different methods; namely, inoculation of the pathogen on the mushroom pileus (IMP) and on the spawned substrate (IMSS) under controlled conditions. Out of the 97 strains screened, 22 P. pulmonarius, and four P. cf. floridanus were moderately resistant to BBD using the IMP method. Eleven P. pulmonarius, six P. cf. florida, and one P. ostreatus strains were highly resistant to BBD using the IMSS method. All of the 97 strains showed varying degrees of susceptibility using the IMP method, but eight strains were completely resistant using the IMSS method. Combining these two methods, five strains were highly resistant (four P. pulmonarius and one P. cf. floridanus) and 11 were moderately resistant (eight P. pulmonarius and three P. cf. floridanus). The resistance sources to P. tolaasii identified in P. pulmonarius and P. cf. floridanus could be used for further breeding of Pleurotus spp.
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