An Interplay between Mitochondrial and ER Targeting of a Bacterial Signal Peptide in Plants.
An Interplay between Mitochondrial and ER Targeting of a Bacterial Signal Peptide in Plants.
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DOI：
10.3390/plants12030617
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发表时间：
2023-01-31
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--
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蛋白质靶向在真核细胞中是维持细胞功能和细胞器特性所必需的。信号肽是一种主要类型的靶向序列，含有三重结构，其在生命的所有结构域中是保守的。它们经常被包括在植物的重组蛋白设计中，以通过将它们定向到内质网（ER）或质外体来增加产量。植物细胞对细菌信号肽的加工还不是很清楚，但可以帮助设计有效的异源表达系统。在这里，我们分析了来自甲烷氧化细菌的酶PmoB的信号肽。在植物细胞中，PmoB信号肽将蛋白质靶向线粒体和ER。在线粒体靶向效率增强的信号肽序列的突变版本中仍然观察到这种双重定位。线粒体靶向被证明是依赖于参与运输到ER的疏水区域。因此，我们认为，双重定位可能是由于ER-SURF途径最近在酵母中的特点。因此，这项工作揭示了植物细胞对细菌信号肽的加工，并提出了植物线粒体靶向的新途径。
Protein targeting is essential in eukaryotic cells to maintain cell function and organelle identity. Signal peptides are a major type of targeting sequences containing a tripartite structure, which is conserved across all domains in life. They are frequently included in recombinant protein design in plants to increase yields by directing them to the endoplasmic reticulum (ER) or apoplast. The processing of bacterial signal peptides by plant cells is not well understood but could aid in the design of efficient heterologous expression systems. Here we analysed the signal peptide of the enzyme PmoB from methanotrophic bacteria. In plant cells, the PmoB signal peptide targeted proteins to both mitochondria and the ER. This dual localisation was still observed in a mutated version of the signal peptide sequence with enhanced mitochondrial targeting efficiency. Mitochondrial targeting was shown to be dependent on a hydrophobic region involved in transport to the ER. We, therefore, suggest that the dual localisation could be due to an ER-SURF pathway recently characterised in yeast. This work thus sheds light on the processing of bacterial signal peptides by plant cells and proposes a novel pathway for mitochondrial targeting in plants.
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