Minute-scale detection of SARS-CoV-2 using a low-cost biosensor composed of pencil graphite electrodes.
Minute-scale detection of SARS-CoV-2 using a low-cost biosensor composed of pencil graphite electrodes.
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DOI：
10.1073/pnas.2106724118
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发表时间：
2021-07-27
影响因子：
11.1
通讯作者：
de la Fuente-Nunez C
中科院分区：
文献类型：
--
作者：
de Lima LF;Ferreira AL;Torres MDT;de Araujo WR;de la Fuente-Nunez C
为了防止COVID-19的传播，迫切需要进行高频率的检测。在这里，我们介绍低成本电化学高级诊断（LEAD），一种诊断测试，检测SARS冠状病毒-2在6.5分钟内，成本为1.50美元每单位，并使用易于组装的材料，如人血管紧张素转换酶2，改性石墨导线，和塑料瓶。当使用临床唾液（100.0%灵敏度、100.0%特异性、100.0%准确度）和鼻咽/口咽（88.7%灵敏度、86.0%特异性、87.4%准确度）样本进行检测时，LEAD的灵敏度与金标准方法（病毒刺突蛋白的检测限= 229 fg·mL-1）相当，并显示出优异的性能特征。最后，未检测到与其他病毒的交叉反应性，并且当在4 °C下储存时，该测试显示出5天的有效保质期。COVID-19已导致全球超过347万人死亡，并继续主要影响中低收入国家。高频率检测已被提议作为预防疫情的潜在解决方案。然而，目前的测试还不够低成本、快速或可扩展，无法进行广泛的COVID-19测试。在这里，我们描述了LEAD（低成本电化学高级诊断），这是一种诊断测试，可在6.5分钟内检测到严重急性呼吸综合征冠状病毒2（SARS-CoV-2），每单位成本为1.50美元，使用易于获得的商业可用材料生产。LEAD对SARS-CoV-2刺突蛋白高度敏感（检测限= 229 fg·mL-1），并在临床唾液（100.0%灵敏度，100.0%特异性和100.0%准确性）和鼻咽/口咽（88.7%灵敏度，86.0%特异性和87.4%准确性）样本中显示出优异的性能。与其他冠状病毒或流感病毒株未检测到交叉反应。重要的是，LEAD还成功地诊断出了高度传染性的SARS-CoV-2 B.1.1.7 UK变体。该装置在所有测试条件下均具有高重现性，并在4 °C磷酸盐缓冲盐水中储存时保持其原始灵敏度5 d。我们的低成本和自助技术开辟了新的途径，以促进高频率的测试，并在资源有限的环境和低收入社区获得急需的诊断测试。
High-frequency testing is urgently needed to help prevent the spread of COVID-19. Here, we introduce Low-cost Electrochemical Advanced Diagnostic (LEAD), a diagnostic test that detects SARS-CoV-2 within 6.5 min, costs $1.50 per unit, and uses easily assembled materials such as human angiotensin-converting enzyme 2, modified graphite leads, and a plastic vial. LEAD presents sensitivity comparable to the gold-standard methods (limit of detection for the viral spike protein = 229 fg⋅mL-1) and displays an excellent performance profile when tested using clinical saliva (100.0% sensitivity, 100.0% specificity, 100.0% accuracy) and nasopharyngeal/oropharyngeal (88.7% sensitivity, 86.0% specificity, 87.4% accuracy) samples. Finally, no cross-reactivity with other viruses was detected and the test displayed a viable shelf-life of 5 d when stored at 4 °C. COVID-19 has led to over 3.47 million deaths worldwide and continues to devastate primarily middle- and low-income countries. High-frequency testing has been proposed as a potential solution to prevent outbreaks. However, current tests are not sufficiently low-cost, rapid, or scalable to enable broad COVID-19 testing. Here, we describe LEAD (Low-cost Electrochemical Advanced Diagnostic), a diagnostic test that detects severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) within 6.5 min and costs $1.50 per unit to produce using easily accessible and commercially available materials. LEAD is highly sensitive toward SARS-CoV-2 spike protein (limit of detection = 229 fg⋅mL−1) and displays an excellent performance profile using clinical saliva (100.0% sensitivity, 100.0% specificity, and 100.0% accuracy) and nasopharyngeal/oropharyngeal (88.7% sensitivity, 86.0% specificity, and 87.4% accuracy) samples. No cross-reactivity was detected with other coronavirus or influenza strains. Importantly, LEAD also successfully diagnosed the highly contagious SARS-CoV-2 B.1.1.7 UK variant. The device presents high reproducibility under all conditions tested and preserves its original sensitivity for 5 d when stored at 4 °C in phosphate-buffered saline. Our low-cost and do-it-yourself technology opens new avenues to facilitate high-frequency testing and access to much-needed diagnostic tests in resource-limited settings and low-income communities.
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影响因子：
1.9
作者：
Fong, Y.;Wakefield, J.;Frahm, N.
通讯作者：
Frahm, N.
DOI：
10.1073/pnas.2014739117
发表时间：
2020-09-15
影响因子：
11.1
作者：
Ganguli A;Mostafa A;Berger J;Aydin MY;Sun F;Ramirez SAS;Valera E;Cunningham BT;King WP;Bashir R
通讯作者：
Bashir R
影响因子：
2.9
作者：
Gottschalk, PG;Dunn, JR
通讯作者：
Dunn, JR
DOI：
10.1016/j.saa.2019.117960
发表时间：
2020-03-15
影响因子：
4.4
作者：
Farmani, Maryam Rahmati;Peyman, Hossein;Roshanfekr, Hamideh
通讯作者：
Roshanfekr, Hamideh
影响因子：
3.1
作者：
Asadzadeh-Firouzabadi, Amineh;Zare, Hamid R.
通讯作者：
Zare, Hamid R.