Insights Into the Role of Exposed Surface Charged Residues in the Alkali-Tolerance of GH11 Xylanase
Insights Into the Role of Exposed Surface Charged Residues in the Alkali-Tolerance of GH11 Xylanase
复制标题
深入了解暴露的表面带电残基在 GH11 木聚糖酶耐碱性中的作用
DOI：
10.3389/fmicb.2020.00872
复制
发表时间：
2020-05
影响因子：
5.2
通讯作者：
Wang Lushan
中科院分区：
文献类型：
--
作者：
Wu Xiuyun;Zhang Qun;Zhang Lanzeng;Liu Shijia;Chen Guanjun;Zhang Huaiqiang;Wang Lushan
热稳定和碱或酸稳定的木聚糖酶在农业和工业领域中更有优势。本研究以嗜热菌GH 11木聚糖酶TlXynA为出发菌株，进行了基于结构的合理设计，以提高其pH耐受性。通过使用定点诱变取代表面带电残基组合来构建四种突变酶（P1、P2、P3和P4）和五种变体（N1、N2、N3、N4和N5）。与天然酶相比，突变体P1和P2表现出更强的耐酸性，特别是在pH3.0时，其最大酶活分别为天然酶的50%和40%。另外，N1、N2、N3和N4四个突变体对碱性环境（pH7.0 -9.0）的耐受性比天然酶高。在pH9.0时，N1、N2、N3和N4的剩余活性分别为86%、78%、77%和66%。总之，一个改进的pH值公差的设计原则正在报告。
Thermostable and alkaline- or acid-stable xylanases are more advantageous in agricultural and industrial fields. In this study, a rational structure-based design was conducted based on a thermostable GH11 xylanase TlXynA from Thermomyces lanuginosus to improved pH-tolerance. Four mutant enzymes (P1, P2, P3, and P4) and five variants (N1, N2, N3, N4, and N5) were constructed by substituting surface charged residue combinations using site-directed mutagenesis. Compared to the native enzyme, two mutants P1 and P2 showed higher acid tolerance, especially at pH 3.0, presented 50 and 40% of their maximum activity, respectively. In addition, four mutants N1, N2, N3 and N4 had higher tolerance than the native enzyme to alkaline environments (pH 7.0–9.0). At pH 9.0, the residual activities of N1, N2, N3, and N4 were 86, 78, 77, and 66%, respectively. In summary, an improved pH-tolerance design principle is being reported.
登录
查看更多内容
影响因子：
4.6
作者：
van Munster JM;Thomas B;Riese M;Davis AL;Gray CJ;Archer DB;Flitsch SL
通讯作者：
Flitsch SL
影响因子：
1.8
作者：
Palaniappan, Ayyappan;Balasubramaniam, V. Geetha;Antony, Usha
通讯作者：
Antony, Usha
影响因子：
5.6
作者：
M. Torruella;M. Flawiá;C. Eisenschlos;L. M. Vedia;C. Rubinstein;H. Torres
通讯作者：
M. Torruella;M. Flawiá;C. Eisenschlos;L. M. Vedia;C. Rubinstein;H. Torres
影响因子：
4.8
作者：
Cheng, Ya-Shan;Chen, Chun-Chi;Guo, Rey-Ting
通讯作者：
Guo, Rey-Ting
DOI：
10.1093/protein/15.2.141
发表时间：
2002-02-01
期刊：
PROTEIN ENGINEERING
影响因子：
--
作者：
Turunen, O;Vuorio, M;Leisola, M
通讯作者：
Leisola, M