Culture optimization for the emergent zooplanktonic model organism Oikopleura dioica.
Culture optimization for the emergent zooplanktonic model organism Oikopleura dioica.
复制标题
DOI：
10.1093/plankt/fbn132
复制
发表时间：
2009-04
影响因子：
2.1
通讯作者：
Thompson EM
中科院分区：
文献类型：
--
作者：
Bouquet JM;Spriet E;Troedsson C;Otterå H;Chourrout D;Thompson EM
泛全球海洋附肢动物Oikopleura dioica在从脊索遗传学和进化到分子生态学研究的跨学科研究中显示出相当大的潜力作为候选模式生物。这种尾索动物具有简化的解剖结构，在不到1周的异常短暂的生命周期中保持透明，并显示出高繁殖力。70Mb，紧凑，测序的基因组是已知最小的后生动物基因组之一，基因调节区和内含子区域的大小都大大减少。生物在海洋生态系统中具有重要的营养作用，是全球垂直碳通量的重要贡献者。在真正的生物模式生物名单中，所有的生物都具有这样的特性，即它们可以在实验室培养中长期维护。在这里，我们测试了饲料制度、产卵密度和稀释度以及海水处理，从而优化了详细的培养方案，允许可持续地长期维持Dioica，允许连续、不间断地生产用于实验的来源材料。培养方案可以在沿海和内陆实验室迅速采用，并应促进该动物提供的许多原创研究视角的快速发展。
The pan-global marine appendicularian, Oikopleura dioica, shows considerable promise as a candidate model organism for cross-disciplinary research ranging from chordate genetics and evolution to molecular ecology research. This urochordate, has a simplified anatomical organization, remains transparent throughout an exceptionally short life cycle of less than 1 week and exhibits high fecundity. At 70 Mb, the compact, sequenced genome ranks among the smallest known metazoan genomes, with both gene regulatory and intronic regions highly reduced in size. The organism occupies an important trophic role in marine ecosystems and is a significant contributor to global vertical carbon flux. Among the short list of bona fide biological model organisms, all share the property that they are amenable to long-term maintenance in laboratory cultures. Here, we tested diet regimes, spawn densities and dilutions and seawater treatment, leading to optimization of a detailed culture protocol that permits sustainable long-term maintenance of O. dioica, allowing continuous, uninterrupted production of source material for experimentation. The culture protocol can be quickly adapted in both coastal and inland laboratories and should promote rapid development of the many original research perspectives the animal offers.
登录
查看更多内容
影响因子：
4.5
作者：
ALLDREDGE, AL
通讯作者：
ALLDREDGE, AL
影响因子：
4.6
作者：
Bassham, S;Postlethwait, JH
通讯作者：
Postlethwait, JH
影响因子：
3.3
作者：
BROWN, MR;FARMER, CL
通讯作者：
FARMER, CL
影响因子：
4.5
作者：
JAMES, CM;ALHINTY, S;SALMAN, AE
通讯作者：
SALMAN, AE
影响因子：
64.8
作者：
Delsuc, F;Brinkmann, H;Philippe, H
通讯作者：
Philippe, H