Robotic RNA extraction for SARS-CoV-2 surveillance using saliva samples.
Robotic RNA extraction for SARS-CoV-2 surveillance using saliva samples.
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DOI：
10.1371/journal.pone.0255690
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
IGI SARS-CoV-2 Consortium
中科院分区：
文献类型：
--
作者：
Hamilton JR;Stahl EC;Tsuchida CA;Lin-Shiao E;Tsui CK;Pestal K;Gildea HK;Witkowsky LB;Moehle EA;McDevitt SL;McElroy M;Keller A;Sylvain I;Hirsh A;Ciling A;Ehrenberg AJ;Ringeisen BR;Huberty G;Urnov FD;Giannikopoulos P;Doudna JA;IGI SARS-CoV-2 Consortium
Saliva is an attractive specimen type for asymptomatic surveillance of COVID-19 in large populations due to its ease of collection and its demonstrated utility for detecting RNA from SARS-CoV-2. Multiple saliva-based viral detection protocols use a direct-to-RT-qPCR approach that eliminates nucleic acid extraction but can reduce viral RNA detection sensitivity. To improve test sensitivity while maintaining speed, we developed a robotic nucleic acid extraction method for detecting SARS-CoV-2 RNA in saliva samples with high throughput. Using this assay, the Free Asymptomatic Saliva Testing (IGI FAST) research study on the UC Berkeley campus conducted 11,971 tests on supervised self-collected saliva samples and identified rare positive specimens containing SARS-CoV-2 RNA during a time of low infection prevalence. In an attempt to increase testing capacity, we further adapted our robotic extraction assay to process pooled saliva samples. We also benchmarked our assay against nasopharyngeal swab specimens and found saliva methods require further optimization to match this gold standard. Finally, we designed and validated a RT-qPCR test suitable for saliva self-collection. These results establish a robotic extraction-based procedure for rapid PCR-based saliva testing that is suitable for samples from both symptomatic and asymptomatic individuals.
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DOI：
10.1016/j.medj.2020.12.010
发表时间：
2021-03-12
期刊：
Med (New York, N.Y.)
影响因子：
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作者：
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通讯作者：
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DOI：
10.3390/v12111314
发表时间：
2020-11-17
期刊：
Viruses
影响因子：
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影响因子：
11.8
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影响因子：
11.8
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Byrne RL;Kay GA;Kontogianni K;Aljayyoussi G;Brown L;Collins AM;Cuevas LE;Ferreira DM;Fraser AJ;Garrod G;Hill H;Hughes GL;Menzies S;Mitsi E;Owen SI;Patterson EI;Williams CT;Hyder-Wright A;Adams ER;Cubas-Atienzar AI
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影响因子：
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通讯作者：
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