Profiling of mouse macrophage lipidome using direct infusion shotgun mass spectrometry.
Profiling of mouse macrophage lipidome using direct infusion shotgun mass spectrometry.
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DOI：
10.1016/j.xpro.2020.100235
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发表时间：
2021-03-19
期刊：
影响因子：
--
通讯作者：
Bensinger SJ
中科院分区：
文献类型：
--
作者：
Hsieh WY;Williams KJ;Su B;Bensinger SJ
免疫细胞，如巨噬细胞，响应于模式识别受体（例如，TLR，NLR）和细胞因子受体（例如，干扰素、白细胞介素）。分析这些变化可以用鸟枪质谱法实现。本方案提供了有关骨髓源性巨噬细胞（BMDM）的生成和刺激、样本收集和脂质提取的分步说明，用于分析巨噬细胞脂质组。有关本方案使用和执行的完整详细信息，请参见）。使用直接输注质谱分析小鼠巨噬细胞脂质组的方案提供免疫细胞脂质组成的定量测量包括细胞培养、细胞成像、样品制备和数据输出分析可适用于不同的脂质组学-质谱平台免疫细胞，如巨噬细胞，响应于模式识别受体（例如，TLR，NLR）和细胞因子受体（例如，干扰素、白细胞介素）。分析这些变化可以用鸟枪质谱法实现。本方案提供了有关骨髓源性巨噬细胞（BMDM）的生成和刺激、样本收集和脂质提取的分步说明，用于分析巨噬细胞脂质组。
Immune cells, such as macrophages, reprogram their lipid metabolism in response to the activation of pattern recognition receptors (e.g., TLRs, NLRs) and cytokine receptors (e.g., interferons, interleukins). Profiling these changes can be achieved with shotgun mass spectrometry. This protocol provides step-by-step instructions on the generation and stimulation of bone marrow-derived macrophages (BMDMs), sample collection, and lipid extraction for profiling the macrophage lipidome. For complete details on the use and execution of this protocol, please refer to). Protocol for profiling mouse macrophage lipidome with direct infusion mass spectrometry Provides quantitative measurements of immune cell lipid composition Includes cell culture, cell imaging, sample preparation, and data output analysis Can be adapted for different lipidomics-mass spectrometry platforms Immune cells, such as macrophages, reprogram their lipid metabolism in response to the activation of pattern recognition receptors (e.g., TLRs, NLRs) and cytokine receptors (e.g., interferons, interleukins). Profiling these changes can be achieved with shotgun mass spectrometry. This protocol provides step-by-step instructions on the generation and stimulation of bone marrow-derived macrophages (BMDMs), sample collection, and lipid extraction for profiling the macrophage lipidome.
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