Mass spectrometry-based shotgun lipidomics - a critical review from the technical point of view.

Mass spectrometry-based shotgun lipidomics - a critical review from the technical point of view.
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DOI:
10.1007/s00216-018-1252-y
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发表时间:
2018-10
影响因子:
4.3
通讯作者:
Hsu FF
Hsu FF
中科院分区:
化学2区
文献类型:
--
作者:
Hsu FF

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在过去的十年中,基于质谱仪(MS)的“鸟枪式脂质组学”已经成为对生物系统中的复杂脂质进行定量和定性分析的有力工具。这篇评论的目的是让感兴趣的读者对该技术的当前状态有一个简明的概述,重点是通过质谱学进行脂肪组学分析。讨论了每种可用的“鸟枪式脂质组学”方法的优缺点和缺陷,并描述了改进现有方法的新策略。还汇编了一份重要论文和综述的清单,这些论文和综述是充分的,但不全面,涵盖了脂质组学的所有方面,包括工作流程、方法学和基本原理,供读者遵循。
Over the past decade, mass spectrometry (MS)-based “shotgun lipidomics” has emerged as a powerful tool for quantitative and qualitative analysis of the complex lipids in the biological system. The aim of this critical review is to give the interested reader a concise overview over the current state of the technology, focused on lipidomic analysis by mass spectrometry. The pros and cons, and pitfalls associated with each available “shotgun lipidomics” method are discussed; and the new strategies for improving the current methods are described. A list of important papers and reviews that are sufficient rather than comprehensive, covering all the aspects of lipidomics including the workflow, methodology, and fundamentals is also compiled for readers to follow.
通过质谱法对组织中脂质生物化学的MALDI成像。
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