Disruption of mesoderm and axis formation in fish by ectopic expression of activin variants: the role of maternal activin.
Disruption of mesoderm and axis formation in fish by ectopic expression of activin variants: the role of maternal activin.
复制标题
激活素变体的异位表达破坏鱼的中胚层和轴形成：母体激活素的作用。
作者：
J. Wittbrodt;F. Rosa
非洲爪蟾胚胎中胚层的形成是涉及肽如FGF或激活素的诱导事件的结果。最近通过截短的激活素受体的异位表达证实，激活素受体信号通路参与体内中胚层和轴形成的过程。然而，这种方法并没有直接解决激活素本身的作用，也没有涉及激活素在其他脊椎动物胚胎中形成中胚层。此外，激活素作为卵的蛋白质组分在母体中表达，并在合子中转录，目前尚不清楚这两种形式中的哪一种参与中胚层的形成。为了解决这三个问题，我们分析了激活素在鱼胚胎发育中的作用，通过产生两个激活素显性阴性变体。其中一种变体作为激活素蛋白的抑制剂。发现第二种变体在与野生型激活素共翻译时耗尽激活素库。注射的RNA编码这些变体的小硬骨鱼Oryzias latipes（日本青）的两细胞胚胎表明，只有母体提供的激活素蛋白是必需的中胚层和轴在鱼体内的形成。
Formation of mesoderm in Xenopus embryos is the result of an induction event in which peptides such as FGF or activins have been implicated. It was recently demonstrated, by the ectopic expression of a truncated activin receptor, that activin receptor signaling pathways are involved in the processes of mesoderm and axis formation in vivo. However, this approach does not directly address the role of activin itself nor the involvement of activins in the formation of mesoderm in embryos from other vertebrates. In addition, activins are expressed maternally as a protein component of the egg as well as transcribed zygotically, and it is not clear which of the two forms is involved in mesoderm formation. To address those three issues, we analyzed the role of activins in the development of fish embryos by generating two activin dominant-negative variants. One of the variants behaves as an inhibitor of activin protein. The second variant was found to deplete the activin pool when cotranslated with wild-type activin. Injection of RNA encoding these variants into the two-cell embryo of the small teleost fish Oryzias latipes (Japanese medaka) demonstrates that only the maternally provided activin protein is required for mesoderm and axis formation in fish in vivo.
影响因子：
4.6
作者：
C. M. Jones;K. Lyons;Peter M Lap An;C. V. Wright;Brigid L. M. Hogan
通讯作者：
C. M. Jones;K. Lyons;Peter M Lap An;C. V. Wright;Brigid L. M. Hogan
影响因子：
4.6
作者：
C. Kimmel;R. M. Warga;T. Schilling
通讯作者：
C. Kimmel;R. M. Warga;T. Schilling