Effect of lycium barbarum (wolfberry) polysaccharides on preserving retinal function after partial optic nerve transection.
Effect of lycium barbarum (wolfberry) polysaccharides on preserving retinal function after partial optic nerve transection.
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DOI：
10.1371/journal.pone.0081339
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Chan HH
中科院分区：
文献类型：
--
作者：
Chu PH;Li HY;Chin MP;So KF;Chan HH
枸杞多糖（LBP）是枸杞（一种传统中药）的活性成分，枸杞长期以来被用于改善视觉功能。本研究旨在探讨部分视神经切断（PONT）模型中视网膜功能的局部变化，以及LBP对视觉功能的影响。从30只斯普拉格 - 道利大鼠的30只眼睛获取多焦视网膜电图（mfERG）。将大鼠分为6组（5个治疗组和1个对照组）。从实验第一天开始，（PONT + LBP）组和（LBP）组的大鼠给予LBP；（PONT + PBS（磷酸盐缓冲盐水））组和（PBS）组的大鼠每天通过鼻胃管给予PBS，直至处死。在第1周结束时（开始给予LBP或PBS后第7天），（PONT）、（PONT + LBP）和（PONT + PBS）组的大鼠视神经背侧部分被切断。在3个时间点测量mfERG：第2周、第3周和第5周。PONT一周后，在所有视网膜区域均观察到mfERG的P1和PhNR振幅显著降低。在PONT之前给予LBP可保护视网膜功能。在上部视网膜中，所有mfERG反应均恢复到正常范围，该区域对应于视神经切断的背侧区域，而在PONT后第4周，大部分下部视网膜反应显著增强。视网膜腹侧部分发生继发性退变，这种退变不仅局限于神经节细胞层，而是一种影响外层视网膜的广泛效应。LBP通过调节来自外层视网膜的信号，改变了由PONT引起的功能降低。
Lycium Barbarum Polysaccharides (LBP) are the active components of Wolfberry (a traditional Chinese medicine) which has long been used for improving visual function. This study aims to investigate localized changes of retinal function in a partial optic nerve transection (PONT) model, and effects of LBP on visual function. The multifocal electroretinograms (mfERG) were obtained from 30 eyes of 30 Sprague-Dawley rats. The rats were divided into 6 groups (five treatment groups and one control group). Starting from the first day of the experiment, the rats in the (PONT+LBP) group and the (LBP) group were dosed with LBP; rats in the (PONT+PBS (phosphate buffered saline)) group and the (PBS) group were dosed with PBS via nasogastric tube every day until euthanized. The dorsal part of the optic nerve was transected in the (PONT), (PONT+LBP) and (PONT+PBS) groups at the end of week 1 (day 7 after LBP or PBS feeding began). The mfERG was measured at three time points: week 2, week 3 and week 5. Significant reduction of P1 and PhNR amplitudes of the mfERG were observed in all retinal regions a week after PONT. Feeding with LBP prior to PONT preserved retinal function. All mfERG responses returned to the normal range in the superior retina, which corresponds to the transected dorsal region of the optic nerve, while most of the inferior retinal responses were significantly increased at week 4 after PONT. The ventral part of the retina had secondary degeneration which was not only limited to the ganglion cell layer, but is a widespread effect affecting the outer retina. LBP altered the functional reduction caused by PONT by regulating the signal from the outer retina.
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