Phytotracker, an information management system for easy recording and tracking of plants, seeds and plasmids.
Phytotracker, an information management system for easy recording and tracking of plants, seeds and plasmids.
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DOI：
10.1186/1746-4811-8-43
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发表时间：
2012-10-13
期刊：
影响因子：
5.1
通讯作者：
Murray JA
中科院分区：
文献类型：
--
作者：
Nieuwland J;Sornay E;Marchbank A;de Graaf BH;Murray JA
在一个典型的植物研究实验室中，大量不同的植物品系被生产和维护，作为种子储备和活跃生长。这些收集需要仔细和一致的管理，以适当地跟踪和维护它们，由于监管要求，这是从事涉及基因操作研究的实验室的一个特别紧迫的问题。研究人员和pi需要访问这些数据和收集，因此，一个易于使用的以植物为导向的实验室信息管理系统，以简单和可视化的格式实现，维护和显示信息，将对实验室的日常工作和确保法规遵从性都有很大帮助。在这里，我们介绍“植物追踪器”，这是一个实验室管理系统，专门用于组织和跟踪可在混合平台环境中使用的质粒、种子和生长植物。Phytotracker的设计以用户操作简单、易于安装和管理为主要因素，同时提供涵盖分子遗传学实验室全部活动的跟踪工具。它利用跨平台的Filemaker关系数据库，这使得它可以作为一个独立运行，或者作为一个基于服务器的网络解决方案，可以在实验室的所有工作站上运行，如果需要，可以通过互联网访问。它也可以很容易地修改或定制进一步。Phytotracker为质粒、种子批次、种子库存和盆栽或托盘中的植物提供编目和搜索功能，并允许从播种、收获到新种子批次的每一株植物进行跟踪，并可以在每个阶段打印适当的标签。该系统从之前的收获数据中输入种子信息，并允许定义和跟踪自交和杂交（杂交）。转基因系可以与它们的质粒DNA来源联系起来。这种易用性和灵活性有助于用户减少组织植物、种子和质粒所需的时间，并保持涉及多名工作人员的实验室连续性。我们已经开发和使用了五年多的Phytotracker，并发现它是一个直观，强大和灵活的研究工具，可以组织我们的质粒，种子和植物收集，需要最少的维护和用户培训。它是在拟南芥分子遗传学环境中开发的，但可以很容易地适应几乎任何植物实验室研究。
A large number of different plant lines are produced and maintained in a typical plant research laboratory, both as seed stocks and in active growth. These collections need careful and consistent management to track and maintain them properly, and this is a particularly pressing issue in laboratories undertaking research involving genetic manipulation due to regulatory requirements. Researchers and PIs need to access these data and collections, and therefore an easy-to-use plant-oriented laboratory information management system that implements, maintains and displays the information in a simple and visual format would be of great help in both the daily work in the lab and in ensuring regulatory compliance. Here, we introduce ‘Phytotracker’, a laboratory management system designed specifically to organise and track plasmids, seeds and growing plants that can be used in mixed platform environments. Phytotracker is designed with simplicity of user operation and ease of installation and management as the major factor, whilst providing tracking tools that cover the full range of activities in molecular genetics labs. It utilises the cross-platform Filemaker relational database, which allows it to be run as a stand-alone or as a server-based networked solution available across all workstations in a lab that can be internet accessible if desired. It can also be readily modified or customised further. Phytotracker provides cataloguing and search functions for plasmids, seed batches, seed stocks and plants growing in pots or trays, and allows tracking of each plant from seed sowing, through harvest to the new seed batch and can print appropriate labels at each stage. The system enters seed information as it is transferred from the previous harvest data, and allows both selfing and hybridization (crossing) to be defined and tracked. Transgenic lines can be linked to their plasmid DNA source. This ease of use and flexibility helps users to reduce their time needed to organise their plants, seeds and plasmids and to maintain laboratory continuity involving multiple workers. We have developed and used Phytotracker for over five years and have found it has been an intuitive, powerful and flexible research tool in organising our plasmid, seed and plant collections requiring minimal maintenance and training for users. It has been developed in an Arabidopsis molecular genetics environment, but can be readily adapted for almost any plant laboratory research.
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影响因子：
4.4
作者：
Melo, Alexandre;Faria-Campos, Alessandra;Campos, Sergio
通讯作者：
Campos, Sergio
影响因子：
3
作者：
Jayashree, B.;Reddy, Praveen T.;Hoisington, David A.
通讯作者：
Hoisington, David A.
影响因子：
3
作者：
Truong, Cong V. C.;Groeneveld, Linn F.;Groeneveld, Eildert
通讯作者：
Groeneveld, Eildert
影响因子：
5.1
作者：
Exner V;Hirsch-Hoffmann M;Gruissem W;Hennig L
通讯作者：
Hennig L