Deep sequencing evidence from single grapevine plants reveals a virome dominated by mycoviruses.
Deep sequencing evidence from single grapevine plants reveals a virome dominated by mycoviruses.
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DOI：
10.1007/s00705-010-0869-8
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发表时间：
2011-03
影响因子：
2.7
通讯作者：
Rowhani, A.
中科院分区：
文献类型：
--
作者：
Al Rwahnih, M.;Daubert, S.;Urbez-Torres, J. R.;Cordero, F.;Rowhani, A.
我们对从葡萄茎中回收的双链RNA进行了454次高通量测序，从而表征了单株葡萄中的病毒体。分析揭示了一组与真菌病毒相似的大量序列。从单一植物来源中鉴定出26个可能的真菌病毒组。它们代表了所有已知的真菌病毒家族的一半，包括金黄色病毒科、副病毒科、纳纳维里科、部分病毒科和环状病毒科。其中三种真菌病毒与葡萄灰霉病菌有关，灰霉病菌是葡萄的一种常见真菌病原。其余的大部分似乎都没有描述。BLAST分析证实了病毒序列的存在，通过使用根据假定的分支病毒基因组序列设计的引物对起始材料产生的PCR产物进行测序来确认存在病毒序列。为了进一步将这些序列鉴定为真菌病毒，对来自葡萄组织的真菌进行了培养，并用相同的PCR探针进行了筛选。在葡萄总提取物中鉴定出的五种分枝病毒在真菌培养物中再次鉴定。本文的在线版本(doi：10.1007/s00705-010869-8)包含补充材料，授权用户可以使用。
We have characterized the virome in single grapevines by 454 high-throughput sequencing of double-stranded RNA recovered from the vine stem. The analysis revealed a substantial set of sequences similar to those of fungal viruses. Twenty-six putative fungal virus groups were identified from a single plant source. These represented half of all known mycoviral families including the Chrysoviridae, Hypoviridae, Narnaviridae, Partitiviridae, and Totiviridae. Three of the mycoviruses were associated with Botrytis cinerea, a common fungal pathogen of grapes. Most of the rest appeared to be undescribed. The presence of viral sequences identified by BLAST analysis was confirmed by sequencing PCR products generated from the starting material using primers designed from the genomic sequences of putative mycoviruses. To further characterize these sequences as fungal viruses, fungi from the grapevine tissue were cultured and screened with the same PCR probes. Five of the mycoviruses identified in the total grapevine extract were identified again in extracts of the fungal cultures. The online version of this article (doi:10.1007/s00705-010-0869-8) contains supplementary material, which is available to authorized users.
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Rowhani, A
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