Middle-aged female rats lack changes in histone H3 acetylation in the anterior hypothalamus observed in young females on the day of a luteinizing hormone surge.
Middle-aged female rats lack changes in histone H3 acetylation in the anterior hypothalamus observed in young females on the day of a luteinizing hormone surge.
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中年雌性大鼠下丘脑前部的组蛋白 H3 乙酰化缺乏在黄体生成素激增当天观察到的年轻雌性大鼠的变化。
DOI：
10.5582/bst.2019.01162
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发表时间：
2019
影响因子：
5.5
通讯作者：
Yan Sun
中科院分区：
文献类型：
--
作者：
Wen Xu;Xiaofei An;N. Zhang;Lisha Li;Xinyan Zhang;Yan Wang;Ling Wang;Yan Sun
组蛋白乙酰化最近被认为与下丘脑的基因转录和雌二醇(E2)的作用有关。本研究旨在确定组蛋白乙酰化在调节雌二醇诱导的黄体生成素(LH)峰中的作用，以了解女性生殖衰老中黄体生成素峰功能障碍的机制。青年和中年雌性大鼠去卵巢(OVX)后给予激素或油治疗，每日1次，连续2天。在预期的促黄体生成素高峰时，采集血液样本进行促黄体生成素测定。分离下丘脑前部和后部，用免疫印迹法检测组蛋白H3/H4乙酰化和组蛋白脱乙酰酶(HDACs)4、-5、-10和-11蛋白表达。我们的结果表明，在年轻女性中，E2显著增加了组蛋白H3的乙酰化，而显著降低了HDAC10蛋白在下丘脑前部的表达。值得注意的是，在中年女性中，雌激素诱导的下丘脑前部组蛋白H3乙酰化和HDAC10的改变是不存在的，这与黄体生成素释放减少有关。然而，AGE改变了下丘脑前部和后部的组蛋白H4乙酰化，并显著增加了下丘脑前部的HDAC4和-5蛋白的表达。综上所述，这些数据表明，组蛋白H3在下丘脑前部的乙酰化可能通过降低HDAC10来调节E2对黄体生成素峰的调节。组蛋白H3乙酰化和HDAC10在下丘脑前部对E2表达的错失反应可能是女性生殖老化时发生的黄体生成素峰失效的原因之一。
Histone acetylation has recently been implicated in gene transcription and estradiol (E2) actions in the hypothalamus. This study aims to determine the involvement of histone acetylation in mediating E2-induced luteinizing hormone (LH) surge to understand the mechanism underlying LH surge dysfunction in female reproductive aging. Young and middle-aged female rats were ovariectomized (OVX) and treated with hormone or oil once per day for two days. At the time of the expected LH surge, blood samples were taken for LH assay. The anterior and posterior hypothalami were dissected, histone H3/H4 acetylation and histone deacetylases (HDACs) 4, -5, -10 and -11 protein expressions were measured using Western blotting. Our results show that in the young females, E2 markedly increased histone H3 acetylation while significantly reducing HDAC10 protein expression in the anterior hypothalamus. Notably, E2-induced alterations of histone H3 acetylation and HDAC10 in the anterior hypothalamus were absent in middle-aged females, associated with a reduced LH release. However, age alters histone H4 acetylation in both the anterior and posterior hypothalamus and significantly increased HDAC 4 and -5 protein expression in the anterior hypothalamus. Taken together, these data suggest that histone H3 acetylation in the anterior hypothalamus may mediate E2 regulation of LH surge and the process possibly through decreasing HDAC10. The missed responsiveness of histone H3 acetylation and HDAC10 expression to E2 in the anterior hypothalamus may contribute to LH surge failure that occurs in female reproductive aging.
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受体三重敲除小鼠评估性类固醇激素的作用。
DOI：
--
发表时间：
2010
期刊：
影响因子：
--
作者：
Matsumoto T.Androgen;Estrogen
通讯作者：
Estrogen
影响因子：
4.8
作者：
Kermath, Bailey A.;Riha, Penny D.;Gore, Andrea C.
通讯作者：
Gore, Andrea C.
影响因子：
3.5
作者：
C. Klinge;S. C. Jernigan;K. Mattingly;K. E. Risinger;J. Zhang
通讯作者：
C. Klinge;S. C. Jernigan;K. Mattingly;K. E. Risinger;J. Zhang
影响因子：
16
作者：
Booth LN;Brunet A
通讯作者：
Brunet A
影响因子：
56.9
作者：
Peleg, Shahaf;Sananbenesi, Farahnaz;Fischer, Andre
通讯作者：
Fischer, Andre