Comparative Evaluation of Soluble and Insoluble-Bound Phenolics and Antioxidant Activity of Two Chinese Mistletoes.
Comparative Evaluation of Soluble and Insoluble-Bound Phenolics and Antioxidant Activity of Two Chinese Mistletoes.
复制标题
两种槲寄生可溶性和不可溶性酚类化合物及抗氧化活性的比较评价
DOI：
10.3390/molecules23020359
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发表时间：
2018-02-08
期刊：
影响因子：
--
通讯作者：
Lu Z
中科院分区：
文献类型：
--
作者：
Li Q;Yang S;Li Y;Xue X;Huang Y;Luo H;Zhang Y;Lu Z
槲寄生可药用以治疗各种人类疾病。有关槲寄生中酚类物质含量及其抗氧化特性的研究报道较少。本研究采用气相色谱-质谱联用技术，测定了茶（Camellia assamica（Mast.）Chang（CMC）和Pyrus，i，f. (CMP)比较了使用高效液相色谱法（HPLC）对CMC和CMP中的酚类化合物进行了鉴定和定量。结果表明，无论在CMC还是CMP中，可溶性酚提取物的TPC均高于不溶性酚提取物。此外，CMP提取的可溶性、不溶性和总酚类组分的TPC（9.91 ± 0.23、4.59 ± 0.27和14.50 ± 0.35 μ mol阿魏酸当量/g干样品）均高于CMC提取的相应值。CMP中可溶性酚类提取物的抗氧化活性高于CMC。18个酚类化合物的可溶性和不溶性结合的酚类提取物的CM进行了鉴定和定量的HPLC。本研究表明，CMC和CMP，特别是后者，可作为抗氧化剂的来源，在人类保健。
Mistletoes are used medicinally in order to treat various human illnesses. Few studies have reported on the phenolic content and antioxidant properties of Chinese mistletoes (CMs). In this work, the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities of soluble and insoluble-bound phenolic extracts from CMs hosted by Camellia assamica (Mast.) Chang (CMC) and Pyrus, i, f. (CMP) were compared. Phenolic compounds in CMC and CMP were identified and quantified using high-performance liquid chromatography (HPLC). The results indicated that the TPC of soluble phenolic extracts was higher than insoluble-bound phenolic counterparts in both CMC and CMP. In addition, the TPC of soluble, insoluble-bound and total phenolic fractions (9.91 ± 0.23, 4.59 ± 0.27 and 14.50 ± 0.35 μmol ferulic acid equivalents per gram (FAE/g) dry sample) extracted from CMP were higher than those extracted from CMC. The soluble phenolic extracts in CMP showed higher antioxidant activities than those in CMC. Eighteen phenolic compounds from soluble and insoluble-bound phenolic extracts from the CMs were identified and quantified by HPLC. This study indicates that CMC and CMP, especially the latter, could be sources of antioxidants in human health care.
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