Reconstruction of 100-year dynamics in Daphnia spawning activity revealed by sedimentary DNA.
Reconstruction of 100-year dynamics in Daphnia spawning activity revealed by sedimentary DNA.
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DOI：
10.1038/s41598-021-03899-0
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发表时间：
2022-02-02
影响因子：
4.6
通讯作者：
Kuwae M
中科院分区：
文献类型：
--
作者：
Tsugeki N;Nakane K;Doi H;Ochi N;Kuwae M
环境DNA（eDNA）是目前研究水生生物种群动态的有力工具。然而，它作为量化评估工具的效用仍在争论中，因为不同物种的eDNA来源并不总是已知的。因此，积累不同物种eDNA来源的信息是迫切需要的。我们的研究目的是评估是否沉积DNA靶向两种水蚤，D。galeata和D. pulicaria，可以跟踪水蚤种群动态和休眠卵产量。应用定量PCR的线粒体12S rRNA基因的沉积物岩心收集在日本的琵琶湖，我们比较了沉积DNA浓度的水蚤与丰富的遗骸和ephippia，反映其丰富度和休眠卵生产，分别。我们发现，在过去的世纪的沉积DNA浓度与它们的人口丰度是不一致的。然而，浓度与休眠卵产量高度相关。我们的研究结果提供了证据，ephippia与休眠卵，产卵活动期间释放，是一个重要的来源，水蚤DNA存档在沉积物中。我们的工作为使用沉积DNA作为监测工具来监测100年前的鸡蛋生产提供了重要的见解。
Environmental DNA (eDNA) is currently developing as a powerful tool for assessing aquatic species dynamics. However, its utility as an assessment tool for quantification remain under debate as the sources of eDNA for different species is not always known. Therefore, accumulating information about eDNA sources from different species is urgently required. The objective of our study was to evaluate whether sedimentary DNA targeting two Daphnia species, D. galeata and D. pulicaria, could track Daphnia population dynamics and resting egg production. Applying a quantitative PCR targeting the mitochondrial 12S rRNA gene on sediment cores collected in Lake Biwa, Japan, we compared sedimentary DNA concentration of Daphnia with the abundance of remains and ephippia, reflecting their abundance and resting egg production, respectively. We found that the sedimentary DNA concentrations of Daphnia for the past century were inconsistent with their population abundance. However, the concentration was highly correlated with the resting egg production. Our results provide evidence that ephippia with resting eggs, released during spawning activities, was a significant source of Daphnia DNA archived in sediments. Our work provides critical insights for using sedimentary DNA as a monitoring tool for egg production dating back 100 years.
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影响因子：
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作者：
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