Development of an immunologically tolerated combination of fluorescent proteins for in vivo two-photon imaging.

Development of an immunologically tolerated combination of fluorescent proteins for in vivo two-photon imaging.
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DOI:
10.1038/srep06664
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发表时间:
2014-10-17
期刊:
影响因子:
4.6
通讯作者:
McGavern DB
McGavern DB
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Gossa S;Nayak D;Zinselmeyer BH;McGavern DB

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荧光蛋白 (FP) 的组合通常用于多参数体内成像实验,以可视化标记的蛋白质或感兴趣的细胞群。涉及 FP 的研究通常受到光谱重叠、毒性、相对量子效率以及转移到不耐受受体后发生免疫排斥的可能性的限制。在这里,我们评估了小鼠免疫系统几种常用 FP 的免疫可见性,并确定了一种光谱兼容、免疫耐受的 FP 组合,非常适合体内双光子成像。
Combinations of fluorescent proteins (FPs) are routinely used for multi-parameter in vivo imaging experiments to visualize tagged proteins or cell populations of interest. Studies involving FPs are often limited by spectral overlap, toxicity, relative quantum efficiency, and the potential for immunological rejection upon transfer into a non-tolerant recipient. Here we evaluate the immunologic visibility of several commonly used FPs by the murine immune system and identify a spectrally compatible, immunologically tolerated combination of FPs well suited for in vivo two-photon imaging.
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