Immunoproteomics to identify species-specific antigens in Neospora caninum recognised by infected bovine sera.
Immunoproteomics to identify species-specific antigens in Neospora caninum recognised by infected bovine sera.
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免疫蛋白质组学以鉴定被感染的牛血清识别的新孢子虫中的物种特异性抗原。
DOI：
10.1051/parasite/2022059
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
牛新孢子虫病是一种令人关注的疾病，因为它在全球范围内分布，并通过乳制品和肉类行业的巨大损失产生重大经济影响。迄今为止，没有有效的化疗药物或疫苗来预防新孢子虫病。因此，这种疾病的控制取决于有效的检测测试，可能会影响治疗管理策略。本研究旨在鉴定犬新孢子虫速殖子在感染牛血清中的特异性免疫反应蛋白。犬、刚地弓形虫、隐孢子虫、牛巴伯虫和B.二联单胞菌，并从未感染的牛血清使用双向凝胶电泳（2-DE）结合免疫印迹和质谱（LC-MS/MS）。在70个蛋白点中，有20个特异性抗原点对应于14种不同的抗原蛋白。犬阳性血清。在这些免疫反应性抗原中，参与细胞增殖和侵袭过程的蛋白质具有高度免疫原性，包括HSP 90样蛋白、推定的微线体4（MIC 4）、肌动蛋白、延伸因子1-α和包含Armadillo/β-catenin-like repeat-containing蛋白。有趣的是，我们发现了一个未知的蛋白质产物，棒状蛋白（ROP 1），具有很强的免疫反应性，对N。犬，但没有可用的功能数据。此外，我们确定了交叉反应抗原之间的这些顶复门寄生虫，特别是N。caninum，T. gondii和C.小的新孢子虫犬特异性免疫显性蛋白的免疫诊断和疫苗开发。这些交叉反应抗原可作为潜在的疫苗候选物或药物靶点，以控制这些顶复门原虫引起的疾病。
Bovine neosporosis is a disease of concern due to its global distribution and significant economic impact through massive losses in the dairy and meat industries. To date, there is no effective chemotherapeutic drug or vaccine to prevent neosporosis. Control of this disease is therefore dependent on efficient detection tests that may affect treatment management strategies. This study was conducted to identify the specific immunoreactive proteins of Neospora caninum tachyzoites recognised by sera from cattle infected with N. caninum, Toxoplasma gondii, Cryptosporidium parvum, Babesia bovis and B. bigemina, and by sera from uninfected cattle using two-DE dimensional gel electrophoresis (2-DE) combined with immunoblot and mass spectrometry (LC-MS/MS). Among 70 protein spots that reacted with all infected sera, 20 specific antigenic spots corresponding to 14 different antigenic proteins were recognised by N. caninum-positive sera. Of these immunoreactive antigens, proteins involved in cell proliferation and invasion process were highly immunogenic, including HSP90-like protein, putative microneme 4 (MIC4), actin, elongation factor 1-alpha and armadillo/beta-catenin-like repeat-containing protein. Interestingly, we discovered an unnamed protein product, rhoptry protein (ROP1), possessing strong immunoreactivity against N. caninum but with no data on function available. Moreover, we identified cross-reactive antigens among these apicomplexan parasites, especially N. caninum, T. gondii and C. parvum. Neospora caninum-specific immunodominant proteins were identified for immunodiagnosis and vaccine development. The cross-reactive antigens could be evaluated as potential common vaccine candidates or drug targets to control the diseases caused by these apicomplexan protozoan parasites.
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影响因子：
4
作者：
Debache, Karim;Guionaud, Christophe;Hemphill, Andrew
通讯作者：
Hemphill, Andrew
DOI：
10.1016/j.jchromb.2004.08.048
发表时间：
2005-02-05
影响因子：
3
作者：
Lee, EG;Kim, JH;Jung, TS
通讯作者：
Jung, TS
影响因子：
4.5
作者：
Cabrera, Ana;Herrmann, Susann;Gilberger, Tim W.
通讯作者：
Gilberger, Tim W.
影响因子：
2.6
作者：
Bartels, Cbxis J. M.;Huinink, Irene;Stegeman, Arjan
通讯作者：
Stegeman, Arjan
影响因子：
2.4
作者：
Liao, M;Xuan, X;Fujisaki, K
通讯作者：
Fujisaki, K