Assignment of the genes for human λ immunoglobulin chains to chromosome 22
Assignment of the genes for human λ immunoglobulin chains to chromosome 22
复制标题
人类 λ 免疫球蛋白链基因分配至 22 号染色体
作者：
J. Erikson;J. Martinis;C. Croce
人免疫球蛋白链在小鼠骨髓瘤细胞和人 B 细胞之间的体细胞杂交体中表达1,2。通过将人免疫球蛋白链的表达与杂交克隆中特定人染色体的存在相关联，我们将人免疫球蛋白重链基因簇分配给人14号染色体（参考文献1,2）。我们现在已经研究了小鼠骨髓瘤细胞与人外周淋巴细胞或不同的人分泌 λ 链的淋巴母细胞系之间的体细胞杂交。体细胞杂交克隆根据其产生人 λ 免疫球蛋白链的能力进行了预先选择，并研究了分配给每个不同人类染色体的同工酶标记的表达。还研究了人类 λ 链分泌杂交克隆的亚克隆。结果表明，人类22号染色体携带人类λ免疫球蛋白链基因。
Human immunoglobulin chains are expressed in somatic cell hybrids between mouse myeloma cells and human B cells1,2. By correlating the expression of human immunoglobulin chains and the presence of specific human chromosomes in the hybrid clones, we have assigned the human immunoglobulin heavy-chain gene cluster to human chromosome 14 (refs 1,2). We have now studied somatic cell hybrids between mouse myeloma cells and either human peripheral lymphocytes or different human λ chain-secreting lymphoblastoid cell lines. Somatic cell hybrid clones were pre-selected for their ability to produce human λ immunoglobulin chains, and studied for the expression of isozyme markers assigned to each of the different human chromosomes. Subclones of human λ chain-secreting hybrid clones were also studied. The results indicate that human chromosome 22 carries the human λ immunoglobulin chain genes.
来自 B 细胞和小鼠-人杂交瘤的人免疫球蛋白 mu 链 cDNA 的克隆和部分核苷酸序列。
DOI：
10.1073/pnas.77.10.6027
发表时间：
1980
影响因子：
11.1
作者：
Dolby,TW;Devuono,J;Croce,CM
通讯作者：
Croce,CM