Bone acidic glycoprotein‐75 self‐associates to form macromolecular complexes in vitro and in vivo with the potential to sequester phosphate ions
Bone acidic glycoprotein‐75 self‐associates to form macromolecular complexes in vitro and in vivo with the potential to sequester phosphate ions
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骨酸性糖蛋白-75 在体外和体内自缔合形成大分子复合物，具有螯合磷酸根离子的潜力
DOI：
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发表时间：
1997
影响因子：
4
通讯作者：
M. McKee
中科院分区：
文献类型：
--
作者：
J. Gorski;E. Kremer;Yan Chen;S. Ryan;Colleen A. Fullenkamp;John Delviscio;Karen Jensen;M. McKee
Monoclonal antibody HTP IV‐#1 specifically recognizes a complexation‐dependent neoepitope on bone acidic glycoprotein‐75 (BAG‐75) and a Mr = 50 kDa fragment. Complexes of BAG‐75 exist in situ, as shown by immunofluorescent staining of the primary spongiosa of rat tibial metaphysis and osteosarcoma cell micromass cultures with monoclonal antibody HTP IV‐#1. Incorporation of BAG‐75 into complexes by newborn growth plate and calvarial tissues was confirmed with a second, anti‐BAG‐75 peptide antibody (#503). Newly synthesized BAG‐75 immunoprecipitated from mineralizing explant cultures of bone was present entirely in large macromolecular complexes, while immunoprecipitates from monolayer cultures of osteoblastic cells were previously shown to contain only monomeric Mr = 75 kDa BAG‐75 and a 50 kDa fragment. Purified BAG‐75 self‐associated in vitro to form large spherical aggregate structures composed of a meshwork of 10 nm diameter fibrils. These structures have the capacity to sequester large amounts of phosphate ions as evidenced by X‐ray microanalysis and by the fact that purified BAG‐75 preparations, even after extensive dialysis against water, retained phosphate ions in concentrations more than 1,000‐fold higher than can be accounted for by exchange calculations or by electrostatic binding. The ultrastructural distribution of immunogold‐labeled BAG‐75 in the primary spongiosa underlying the rat growth plate is distinct from that for other acidic phosphoproteins, osteopontin and bone sialoprotein. We conclude that BAG‐75 self‐associates in vitro and in vivo into microfibrillar complexes which are specifically recognized by monoclonal antibody HTP IV‐#1. This propensity to self‐associate into macromolecular complexes is not shared with acidic phosphoproteins osteopontin and bone sialoprotein. We hypothesize that an extracellular electronegative network of macromolecular BAG‐75 complexes could serve an organizational role in forming bone or as a barrier restricting local diffusion of phosphate ions. J. Cell. Biochem. 64:547–564. © 1997 Wiley‐Liss, Inc.
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DOI：
10.1016/s0021-9258(18)98430-9
发表时间：
1993-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
F. Ross;J. Chappel;Jose I. AlvarezS;Diane Sanderll;W. Butler;M. Farach-Carson;Keith A. Mintz;P. Robey;Steven L. TeitelbaumS;David A. Chereshll
通讯作者：
F. Ross;J. Chappel;Jose I. AlvarezS;Diane Sanderll;W. Butler;M. Farach-Carson;Keith A. Mintz;P. Robey;Steven L. TeitelbaumS;David A. Chereshll
鸡骨中两种磷蛋白的比较及其与哺乳动物骨蛋白骨桥蛋白和骨唾液酸蛋白II的相似性。
DOI：
10.1016/s0006-291x(05)81082-4
发表时间：
1990
影响因子：
3.1
作者：
Gotoh,Y;Pierschbacher,MD;Grzesiak,JJ;Gerstenfeld,L;Glimcher,MJ
通讯作者：
Glimcher,MJ
影响因子：
2.8
作者：
Rapuano, BE;Wu, C;MacDonald, DE
通讯作者：
MacDonald, DE
DOI：
10.1016/s0169-6009(08)80225-5
发表时间：
1993-08-01
期刊：
BONE AND MINERAL
影响因子：
--
作者：
BOSKEY, AL;MARESCA, M;PRINCE, CW
通讯作者：
PRINCE, CW