Effects of putrefaction on the quantitative analyses of diphenhydramine in blood and tissues: model experiments by the routine matrix-matched calibration method and standard addition method with different internal standards
Effects of putrefaction on the quantitative analyses of diphenhydramine in blood and tissues: model experiments by the routine matrix-matched calibration method and standard addition method with different internal standards
复制标题
腐败对血液和组织中苯海拉明定量分析的影响：常规基质匹配校准法和不同内标标准添加法模型实验
DOI：
10.1007/s11419-021-00580-z
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发表时间：
2021
影响因子：
2.2
通讯作者：
Kubo Shin-ichi
中科院分区：
文献类型：
--
作者：
Kudo Keiko;Saka Kanju;Waters Brian;Hara Kenji;Matsusue Aya;Kashiwagi Masayuki;Kubo Shin-ichi
目的探讨常规基质匹配校正(MMC)法和标准加入法(SA)测定血液和组织中苯海拉明时，腐烂对测定结果的影响。在制备的样品中加入中毒量苯海拉明和6个具有不同相似性的内标(ISS)，采用液-液萃取法提取药物。采用高效液相色谱-串联质谱法测定苯海拉明的浓度。比较各定量方法的准确度和精密度。结果在MMC方法中，氚标记的IS法对腐烂的样品具有良好的准确度和精密度。结论采用同位素标记的新鲜血液校正曲线的MMC方法可以准确地测量腐烂血液和组织中的药物浓度。如果没有同位素标记的化合物，用MMC方法进行定量似乎有效地使用了几个相似性很高的ISS。据我们所知，这类关于腐烂对法医分析影响的研究尚未见报道。
PurposeThe purpose of this study was to elucidate the effect of putrefaction on the quantitative analyses of diphenhydramine in blood and tissues by the routine matrix-matched calibration (MMC) method and the standard addition (SA) method for comparison.MethodsPutrefied samples were prepared by leaving pig blood, liver and muscle at room temperature. Toxic amounts of diphenhydramine and six internal standards (ISs) having different similarities were added to the prepared samples, and the drug was extracted with a liquid-liquid extraction method. The concentrations of diphenhydramine were determined by liquid chromatography–tandem mass spectrometry. The accuracies and precisions of the obtained quantitative values were compared in each quantification method. The results were also verified using human blood and tissues obtained at the time of autopsy.ResultsIn the MMC method, the deuterium-labeled IS showed good accuracies and precisions for putrefied samples. On the other hand, the concentrations of diphenhydramine using some non-isotopic ISs were found to deviate significantly from the true value.ConclusionsThe obtained results showed that the MMC method using fresh blood calibration curve with isotope-labeled IS could accurately measure drug concentrations in putrefied blood and tissues. If the isotope-labeled compound is not available, the quantification by the MMC method seems effective using several ISs with high similarity. This kind of study on the effects of putrefaction on forensic analyses has not been reported to our knowledge.
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