The Isolation and In Vitro Differentiation of Primary Fetal Baboon Tracheal Epithelial Cells for the Study of SARS-CoV-2 Host-Virus Interactions.
The Isolation and In Vitro Differentiation of Primary Fetal Baboon Tracheal Epithelial Cells for the Study of SARS-CoV-2 Host-Virus Interactions.
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DOI：
10.3390/v15040862
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发表时间：
2023-03-28
期刊：
影响因子：
--
通讯作者：
Walters MS
中科院分区：
文献类型：
--
作者：
Subramaniyan B;Gurung S;Bodas M;Moore AR;Larabee JL;Reuter D;Georgescu C;Wren JD;Myers DA;Papin JF;Walters MS
粘膜纤毛气道上皮排列在人气道上，是肺中宿主-环境相互作用的主要部位。在病毒感染后，气道上皮细胞启动先天免疫应答以抑制病毒复制。因此，定义病毒-宿主相互作用的粘膜纤毛气道上皮细胞是至关重要的，了解机制，调节病毒感染，包括严重急性呼吸道综合征冠状病毒2（SARS-CoV-2）。非人灵长类动物（NHP）与人类关系密切，为研究人类疾病提供了模型。然而，伦理考虑和高成本可能会限制体内NHP模型的使用。因此，需要开发人呼吸道病毒感染的体外NHP模型，其将允许快速表征病毒嗜性和特定NHP物种对模拟人感染的适用性。使用橄榄狒狒（Papio anubis），我们已经开发出的分离，体外扩增，冷冻保存，和原代胎儿狒狒气管上皮细胞（FBTECs）的粘液纤毛分化的方法。此外，我们证明，在体外分化的FBTECs是允许的SARS-CoV-2感染，并产生一个强大的宿主先天免疫反应。综上所述，我们已经建立了一个体外NHP模型，为研究SARS-CoV-2感染和其他人类呼吸道病毒提供了一个平台。
The mucociliary airway epithelium lines the human airways and is the primary site of host-environmental interactions in the lung. Following virus infection, airway epithelial cells initiate an innate immune response to suppress virus replication. Therefore, defining the virus-host interactions of the mucociliary airway epithelium is critical for understanding the mechanisms that regulate virus infection, including Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Non-human primates (NHP) are closely related to humans and provide a model to study human disease. However, ethical considerations and high costs can restrict the use of in vivo NHP models. Therefore, there is a need to develop in vitro NHP models of human respiratory virus infection that would allow for rapidly characterizing virus tropism and the suitability of specific NHP species to model human infection. Using the olive baboon (Papio anubis), we have developed methodologies for the isolation, in vitro expansion, cryopreservation, and mucociliary differentiation of primary fetal baboon tracheal epithelial cells (FBTECs). Furthermore, we demonstrate that in vitro differentiated FBTECs are permissive to SARS-CoV-2 infection and produce a potent host innate-immune response. In summary, we have developed an in vitro NHP model that provides a platform for the study of SARS-CoV-2 infection and other human respiratory viruses.
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DOI：
10.3390/v14071507
发表时间：
2022-07-09
期刊：
Viruses
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.7
作者：
Broadbent, Lindsay;Bamford, Connor G. G.;Lopez Campos, Guillermo;Manzoor, Sheerien;Courtney, David;Ali, Ahlam;Touzelet, Olivier;McCaughey, Conall;Mills, Ken;Power, Ultan F.
通讯作者：
Power, Ultan F.
影响因子：
3.7
作者：
Hackett NR;Shaykhiev R;Walters MS;Wang R;Zwick RK;Ferris B;Witover B;Salit J;Crystal RG
通讯作者：
Crystal RG
影响因子：
64.8
作者：
Delorey TM;Ziegler CGK;Heimberg G;Normand R;Yang Y;Segerstolpe Å;Abbondanza D;Fleming SJ;Subramanian A;Montoro DT;Jagadeesh KA;Dey KK;Sen P;Slyper M;Pita-Juárez YH;Phillips D;Biermann J;Bloom-Ackermann Z;Barkas N;Ganna A;Gomez J;Melms JC;Katsyv I;Normandin E;Naderi P;Popov YV;Raju SS;Niezen S;Tsai LT;Siddle KJ;Sud M;Tran VM;Vellarikkal SK;Wang Y;Amir-Zilberstein L;Atri DS;Beechem J;Brook OR;Chen J;Divakar P;Dorceus P;Engreitz JM;Essene A;Fitzgerald DM;Fropf R;Gazal S;Gould J;Grzyb J;Harvey T;Hecht J;Hether T;Jané-Valbuena J;Leney-Greene M;Ma H;McCabe C;McLoughlin DE;Miller EM;Muus C;Niemi M;Padera R;Pan L;Pant D;Pe'er C;Pfiffner-Borges J;Pinto CJ;Plaisted J;Reeves J;Ross M;Rudy M;Rueckert EH;Siciliano M;Sturm A;Todres E;Waghray A;Warren S;Zhang S;Zollinger DR;Cosimi L;Gupta RM;Hacohen N;Hibshoosh H;Hide W;Price AL;Rajagopal J;Tata PR;Riedel S;Szabo G;Tickle TL;Ellinor PT;Hung D;Sabeti PC;Novak R;Rogers R;Ingber DE;Jiang ZG;Juric D;Babadi M;Farhi SL;Izar B;Stone JR;Vlachos IS;Solomon IH;Ashenberg O;Porter CBM;Li B;Shalek AK;Villani AC;Rozenblatt-Rosen O;Regev A
通讯作者：
Regev A
影响因子：
8
作者：
Davis JD;Wypych TP
通讯作者：
Wypych TP