Using TIRF microscopy to quantify and confirm efficient mass transfer at the substrate surface of the chemistrode.
Using TIRF microscopy to quantify and confirm efficient mass transfer at the substrate surface of the chemistrode.
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DOI：
10.1088/1367-2630/11/7/075017
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发表时间：
2009
影响因子：
3.3
通讯作者：
Ismagilov RF
中科院分区：
文献类型：
--
作者：
Chen D;Du W;Ismagilov RF
本文描述了在化学计量器中表征基质亲水表面传质的实验。该chemistrode使用微流体塞以高时间分辨率将化学品脉冲递送到基底，这需要基底的润湿层和亲水表面之间的有效传质。在这里，全内反射荧光显微镜（TIRFM）被用来成像的亲水性表面的基板作为塞子，使其流过。基板的表面是迅速饱和的荧光染料作为fluroesecent塞子通过基板，确认有效的质量转移之间的润湿层和基板的表面。饱和的动力学在循环与循环之间是一致的，表明化学物质可以以高再现性刺激表面。达到90%饱和度的亲水性表面的基板上，ESTA（90%）所需的插头的数量，只有微弱的依赖于实验条件（Péclet数或毛细管数）。此外，在宽范围的操作条件下，λ（90%）小于4。这些结果是有用的，以改善chemistrod和了解其他现象，涉及多相或表面附近的再循环流的扩散传递。
This paper describes experiments for characterizing mass transfer at the hydrophilic surface of the substrate in a chemistrode. The chemistrode uses microfluidic plugs to deliver pulses of chemicals to a substrate with high temporal resolution, which requires efficient mass transfer between the wetting layer and the hydrophilic surface of the substrate. Here, total internal reflection fluorescence microscopy (TIRFM) was used to image the hydrophilic surface of the substrate as plugs were made to flow over it. The surface of the substrate was rapidly saturated with a fluorescent dye as the fluroesecent plugs passed over the substrate, confirming effective mass transfer between the wetting layer and the surface of the substrate. The dynamics of saturation are consistent from cycle to cycle, indicating that the chemistrode can stimulate surfaces with high reproducibility. The number of plugs required to reach 90% saturation of the hydrophilic surface of the substrate, ϕ(90%), only weakly depended on experimental conditions (the Péclet number or the capillary number). Furthermore, over a wide range of operating conditions, ϕ(90%) was less than 4. These results are useful for improving the chemistrode and for understanding other phenomena that involve diffusional transfer in multiphase or recirculating flows near surfaces.
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影响因子：
16.6
作者：
Link, DR;Grasland-Mongrain, E;Weitz, DA
通讯作者：
Weitz, DA
DOI：
10.1098/rsta.2003.1356
发表时间：
2004-05-15
期刊：
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES
影响因子：
--
作者：
Wiggins, S;Ottino, JM
通讯作者：
Ottino, JM
影响因子：
7.4
作者：
Lorenz, Robert M.;Edgar, J. Scott;Chiu, Daniel T.
通讯作者：
Chiu, Daniel T.
影响因子：
4
作者：
Ismagilov, RF;Stroock, AD;Stone, HA
通讯作者：
Stone, HA
影响因子：
6.1
作者：
Garstecki, P;Fuerstman, MJ;Whitesides, GM
通讯作者：
Whitesides, GM