MinION Whole-Genome Sequencing in Resource-Limited Settings: Challenges and Opportunities.
MinION Whole-Genome Sequencing in Resource-Limited Settings: Challenges and Opportunities.
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DOI：
10.1007/s40588-022-00183-1
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发表时间：
2022
影响因子：
5.2
通讯作者：
Bazira, Joel
中科院分区：
文献类型：
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作者：
Wasswa, Fredrickson B.;Kassaza, Kennedy;Nielsen, Kirsten;Bazira, Joel
MinION全基因组测序技术的引入大大增加和简化了全球各个科学领域的全基因组测序。从复杂生物体到微生物的序列已经产生，并存储在基因组数据库中，便于研究人员访问。各种用于基因组分析的新软件，以及对旧软件包的升级，正在产生。新的协议也正在得到验证，使WGS技术能够迅速并越来越多地用于现场测序。与其他测序技术相比，MinION WGS技术具有便携性、实时分析和较低成本等优点，已在发达国家实施。虽然这些同样的优势在发展中国家至关重要，但在资源有限的情况下，MinION WGS技术仍未得到充分利用。在这篇综述中，我们着眼于在资源有限的环境中使用MinION WGS的应用、优势、挑战和机遇。
The introduction of MinION whole-genome sequencing technology greatly increased and simplified complete genome sequencing in various fields of science across the globe. Sequences have been generated from complex organisms to microorganisms and are stored in genome databases that are readily accessible by researchers. Various new software for genome analysis, along with upgrades to older software packages, are being generated. New protocols are also being validated that enable WGS technology to be rapidly and increasingly used for sequencing in field settings. MinION WGS technology has been implemented in developed countries due to its advantages: portability, real-time analysis, and lower cost compared to other sequencing technologies. While these same advantages are critical in developing countries, MinION WGS technology is still under-utilized in resource-limited settings. In this review, we look at the applications, advantages, challenges, and opportunities of using MinION WGS in resource-limited settings.
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影响因子：
7.7
作者：
Ekeng E;Tchatchouang S;Akenji B;Issaka BB;Akintayo I;Chukwu C;Dano ID;Melingui S;Ousmane S;Popoola MO;Nzouankeu A;Boum Y;Luquero F;Ahumibe A;Naidoo D;Azman A;Lessler J;Wohl S
通讯作者：
Wohl S
DOI：
10.3390/v12111255
发表时间：
2020-11-04
期刊：
Viruses
影响因子：
--
作者：
Gigante CM;Yale G;Condori RE;Costa NC;Long NV;Minh PQ;Chuong VD;Tho ND;Thanh NT;Thin NX;Hanh NTH;Wambura G;Ade F;Mito O;Chuchu V;Muturi M;Mwatondo A;Hampson K;Thumbi SM;Thomae BG;de Paz VH;Meneses S;Munyua P;Moran D;Cadena L;Gibson A;Wallace RM;Pieracci EG;Li Y
通讯作者：
Li Y
影响因子：
5.2
作者：
Imai K;Tamura K;Tanigaki T;Takizawa M;Nakayama E;Taniguchi T;Okamoto M;Nishiyama Y;Tarumoto N;Mitsutake K;Murakami T;Maesaki S;Maeda T
通讯作者：
Maeda T
影响因子：
4.6
作者：
Giordano F;Aigrain L;Quail MA;Coupland P;Bonfield JK;Davies RM;Tischler G;Jackson DK;Keane TM;Li J;Yue JX;Liti G;Durbin R;Ning Z
通讯作者：
Ning Z
影响因子：
11.8
作者：
Hoenen T;Groseth A;Rosenke K;Fischer RJ;Hoenen A;Judson SD;Martellaro C;Falzarano D;Marzi A;Squires RB;Wollenberg KR;de Wit E;Prescott J;Safronetz D;van Doremalen N;Bushmaker T;Feldmann F;McNally K;Bolay FK;Fields B;Sealy T;Rayfield M;Nichol ST;Zoon KC;Massaquoi M;Munster VJ;Feldmann H
通讯作者：
Feldmann H