RNA interference screening to detect targetable molecules in hematopoietic stem cells
RNA interference screening to detect targetable molecules in hematopoietic stem cells
复制标题
RNA干扰筛选检测造血干细胞中的靶向分子
DOI：
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发表时间：
2014
影响因子：
3.2
通讯作者：
Jonas Larsson
中科院分区：
文献类型：
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作者：
C. Karlsson;Justyna Rak;Jonas Larsson
Purpose of reviewThe molecular principles regulating hematopoietic stem cells (HSCs) remain incompletely defined. In this review, we will discuss how RNA interference (RNAi) screening has emerged as a new and powerful tool to molecularly dissect various functional aspects of both normal and malignant HSCs, and how this may ultimately enable the discovery of novel therapeutic targets for clinical applications. Recent findingsAdvances in RNAi technology and the creation of short hairpin RNA retroviral and lentiviral vector-libraries have provided tools to perform broad forward genetic screens in primary mammalian hematopoietic cells. Recent studies have identified novel fate determinants in murine HSCs as well as potential targets for ex-vivo expansion of human HSCs. RNAi screens have further unraveled tumor suppressor genes associated with hematopoietic neoplasms as well as candidate therapeutic targets in leukemic cells. SummaryRNAi screening is a feasible tool to discover novel molecules that regulate both normal and malignant HSCs, thus increasing our general understanding of the physiology and pathophysiology of hematopoiesis. Moreover, RNAi technology holds great promise for the discovery of specific targets for therapeutic interventions.
影响因子：
50.3
作者：
Miller PG;Al-Shahrour F;Hartwell KA;Chu LP;Järås M;Puram RV;Puissant A;Callahan KP;Ashton J;McConkey ME;Poveromo LP;Cowley GS;Kharas MG;Labelle M;Shterental S;Fujisaki J;Silberstein L;Alexe G;Al-Hajj MA;Shelton CA;Armstrong SA;Root DE;Scadden DT;Hynes RO;Mukherjee S;Stegmaier K;Jordan CT;Ebert BL
通讯作者：
Ebert BL
影响因子：
50.3
作者：
Bric A;Miething C;Bialucha CU;Scuoppo C;Zender L;Krasnitz A;Xuan Z;Zuber J;Wigler M;Hicks J;McCombie RW;Hemann MT;Hannon GJ;Powers S;Lowe SW
通讯作者：
Lowe SW