Crystal Structure of Borrelia turicatae protein, BTA121, a differentially regulated gene in the tick-mammalian transmission cycle of relapsing fever spirochetes.
Crystal Structure of Borrelia turicatae protein, BTA121, a differentially regulated gene in the tick-mammalian transmission cycle of relapsing fever spirochetes.
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DOI：
10.1038/s41598-017-14959-9
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发表时间：
2017-11-10
影响因子：
4.6
通讯作者：
Asojo OA
中科院分区：
文献类型：
--
作者：
Luo Z;Kelleher AJ;Darwiche R;Hudspeth EM;Shittu OK;Krishnavajhala A;Schneiter R;Lopez JE;Asojo OA
蜱传回归热（RF）疏螺旋体病是一种被忽视的疾病，经常被误诊。在美国传播的RF物种包括疏螺旋体，它是由argasid蜱传播的。RF疏螺旋体的环境适应知之甚少，但我们以前的研究表明B的差异调节。在蜱-哺乳动物传播周期中，turiclactinase基因定位于150 kb线性大质粒上，包括BTA 121。该基因被B上调。turiclactin在蜱与哺乳动物中的差异，并且预测编码的蛋白质（BTA 121）是表面定位的。利用硒代蛋氨酸衍生蛋白质，通过单波长反常色散（SAD）解析了BTA 121的结构。BTA 121的拓扑结构是独特的，具有组织成两个螺旋束的四个螺旋结构域。由于巨质粒上几个基因的序列相似性，BTA 121可以作为它们三级结构的模型。BTA 121具有大的相互连接的隧道和空腔，可以容纳配体，特别是长的平行螺旋，其具有大的疏水中心口袋。初步的体外研究表明，BTA 121结合脂质，特别是棕榈酸酯，其结合亲和力与tablysin-15（一种已知的棕榈酸酯结合蛋白）相似。报告的数据将指导机制研究，以确定BTA 121在B的蜱-哺乳动物传播周期中的作用。turiclavia。
Tick-borne relapsing fever (RF) borreliosis is a neglected disease that is often misdiagnosed. RF species circulating in the United States include Borrelia turicatae, which is transmitted by argasid ticks. Environmental adaptation by RF Borrelia is poorly understood, however our previous studies indicated differential regulation of B. turicatae genes localized on the 150 kb linear megaplasmid during the tick-mammalian transmission cycle, including bta121. This gene is up-regulated by B. turicatae in the tick versus the mammal, and the encoded protein (BTA121) is predicted to be surface localized. The structure of BTA121 was solved by single-wavelength anomalous dispersion (SAD) using selenomethionine-derivative protein. The topology of BTA121 is unique with four helical domains organized into two helical bundles. Due to the sequence similarity of several genes on the megaplasmid, BTA121 can serve as a model for their tertiary structures. BTA121 has large interconnected tunnels and cavities that can accommodate ligands, notably long parallel helices, which have a large hydrophobic central pocket. Preliminary in-vitro studies suggest that BTA121 binds lipids, notably palmitate with a similar order of binding affinity as tablysin-15, a known palmitate-binding protein. The reported data will guide mechanistic studies to determine the role of BTA121 in the tick-mammalian transmission cycle of B. turicatae.
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影响因子：
5.8
作者：
Kozlikova, Barbora;Sebestova, Eva;Sochor, Jiri
通讯作者：
Sochor, Jiri
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
影响因子：
5.7
作者：
Kazimírová M;Štibrániová I
通讯作者：
Štibrániová I
DOI：
10.1107/s0907444904026460
发表时间：
2004-12-01
影响因子：
2.2
作者：
Krissinel, E;Henrick, K
通讯作者：
Henrick, K
影响因子：
4.3
作者：
Chovancova E;Pavelka A;Benes P;Strnad O;Brezovsky J;Kozlikova B;Gora A;Sustr V;Klvana M;Medek P;Biedermannova L;Sochor J;Damborsky J
通讯作者：
Damborsky J