Regulation of plant phototropic growth by NPH3/RPT2-like substrate phosphorylation and 14-3-3 binding.
Regulation of plant phototropic growth by NPH3/RPT2-like substrate phosphorylation and 14-3-3 binding.
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DOI：
10.1038/s41467-021-26333-5
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发表时间：
2021-10-21
影响因子：
16.6
通讯作者：
Christie JM
中科院分区：
文献类型：
--
作者：
Sullivan S;Waksman T;Paliogianni D;Henderson L;Lütkemeyer M;Suetsugu N;Christie JM
极性是植物所有定向生长反应的基础，包括向光生长（向光性）。质膜相关蛋白NPH 3是植物向光性生长的一个关键决定因子，受光致蛋白AGC激酶的调控。在这里，我们证明，NPH 3是直接磷酸化的phot 1内的保守的C-末端的共识序列（RXS），这是必要的，以促进向光性和叶柄定位在拟南芥。RxS磷酸化也触发14-3-3结合NPH 3磷酸化和定位状态的变化。不能结合或组成性结合14-3-3 s的NPH 3突变体显示出与模型一致的受损功能，其中向光性曲率是通过跨越茎的NPH 3 RxS磷酸化梯度产生的信号输出建立的。因此，我们的研究结果表明，NPH 3/RPT 2样（NRL）蛋白是植物AGC激酶的磷酸化靶点。此外，RxS磷酸化在NRL家族的其他成员中是保守的，表明调节植物生长以适应主要光环境的共同机制。定位于质膜的NPH 3蛋白是拟南芥向光生长所必需的。在这里，作者表明，向光蛋白1在保守的C-末端序列基序磷酸化NPH 3，触发14-3-3蛋白与NPH 3的结合，这是向光反应所必需的。
Polarity underlies all directional growth responses in plants including growth towards the light (phototropism). The plasma-membrane associated protein, NON-PHOTOTROPIC HYPOCOTYL 3 (NPH3) is a key determinant of phototropic growth which is regulated by phototropin (phot) AGC kinases. Here we demonstrate that NPH3 is directly phosphorylated by phot1 within a conserved C-terminal consensus sequence (RxS) that is necessary to promote phototropism and petiole positioning in Arabidopsis. RxS phosphorylation also triggers 14-3-3 binding combined with changes in NPH3 phosphorylation and localisation status. Mutants of NPH3 that are unable to bind or constitutively bind 14-3-3 s show compromised functionality consistent with a model where phototropic curvature is established by signalling outputs arising from a gradient of NPH3 RxS phosphorylation across the stem. Our findings therefore establish that NPH3/RPT2-Like (NRL) proteins are phosphorylation targets for plant AGC kinases. Moreover, RxS phosphorylation is conserved in other members of the NRL family, suggesting a common mechanism of regulating plant growth to the prevailing light environment. The plasma membrane-localized NPH3 protein is required for phototropic growth in Arabidopsis. Here the authors show that phototropin 1 phosphorylates NPH3 at a conserved C-terminal sequence motif triggering binding of 14-3-3 proteins to NPH3 that is necessary for the phototropic response.
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DOI：
10.1016/j.cub.2021.02.028
发表时间：
2021-05-10
期刊：
Current biology : CB
影响因子：
--
作者：
Glanc M;Van Gelderen K;Hoermayer L;Tan S;Naramoto S;Zhang X;Domjan D;Včelařová L;Hauschild R;Johnson A;de Koning E;van Dop M;Rademacher E;Janson S;Wei X;Molnár G;Fendrych M;De Rybel B;Offringa R;Friml J
通讯作者：
Friml J
影响因子：
16.6
作者：
Hiyama A;Takemiya A;Munemasa S;Okuma E;Sugiyama N;Tada Y;Murata Y;Shimazaki KI
通讯作者：
Shimazaki KI
影响因子：
14.9
作者：
Durek P;Schmidt R;Heazlewood JL;Jones A;MacLean D;Nagel A;Kersten B;Schulze WX
通讯作者：
Schulze WX
影响因子：
4.6
作者：
Furutani, Masahiko;Sakamoto, Norihito;Tasaka, Masao
通讯作者：
Tasaka, Masao
影响因子：
11.6
作者：
Inada, S;Ohgishi, M;Sakai, T
通讯作者：
Sakai, T