Distinct Cytokine and Chemokine Expression in Plasma and Calpeptin-Treated PBMCs of a Relapsing-Remitting Multiple Sclerosis Patient: A Case Report.
Distinct Cytokine and Chemokine Expression in Plasma and Calpeptin-Treated PBMCs of a Relapsing-Remitting Multiple Sclerosis Patient: A Case Report.
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DOI：
10.1007/s11064-018-2655-z
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发表时间：
2018-12
影响因子：
4.4
通讯作者：
Banik, Naren L.
中科院分区：
文献类型：
--
作者：
Chandran, Raghavendar;Capone, Mollie;Matzelle, Denise;Polcyn, Rachel;Kau, Elizabeth;Haque, Azizul;Banik, Naren L.
关键词：
使用两次单独就诊获得的患者血液样本，对一名患有复发缓解型多发性硬化症 (RRMS) 的 60 岁非裔美国男性的细胞因子/趋化因子表达特征进行了分析。使用基于微珠的多重免疫测定法在 RRMS 患者的血浆中检测到 36 种不同的细胞因子、趋化因子和生长因子。结果表明，这些因子中至少有 10 种浓度 > 100 pg/mL 被确定为促炎因子。钙蛋白酶抑制导致抗炎作用，如促炎细胞因子/趋化因子（如 GM-CSF、IFNγ 和 IL-17A）表达减少，以及用抗 CD3/CD28 激活的外周血单核细胞中两种抗炎细胞因子（IL-13 和 IL-4）相对增加所表明的。总体而言，这些结果表明，在 RRMS 患者血浆中观察到的独特细胞因子/趋化因子模式可用作预后标志物，钙蛋白酶抑制可用作治疗 RRMS 患者特有的过度炎症反应的新治疗策略。
The cytokine/chemokine expression signature of a 60-year-old African American male with relapsing-remitting multiple sclerosis (RRMS) was analyzed using patient blood samples obtained from two separate visits to the clinic. Thirty-six different cytokines, chemokines, and growth factors were detected in the plasma of the RRMS patient using a multiplexed bead-based immunoassay. Results indicated that at least ten of these factors with a concentration of > 100 pg/mL are identified as pro-inflammatory. Calpain inhibition led to an anti-inflammatory effect, as indicated by a decrease in expression of pro-inflammatory cytokines/chemokines such as GM-CSF, IFNγ, and IL-17A, and a relative increase in two of the anti-inflammatory cytokines (IL-13 and IL-4) in the peripheral blood mononuclear cells activated with anti-CD3/CD28. Overall, these results suggest that the unique cytokine/chemokine pattern observed in the plasma of the RRMS patient can be used as a prognostic marker and calpain inhibition may be used as a novel therapeutic strategy for treating excessive inflammatory response specific to RRMS patients.
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影响因子：
2.4
作者：
Glabinski, AR;Tuohy, VK;Ransohoff, RM
通讯作者：
Ransohoff, RM
影响因子：
5.1
作者：
Samantaray S;Knaryan VH;Shields DC;Cox AA;Haque A;Banik NL
通讯作者：
Banik NL
影响因子：
9.3
作者：
Bassi, Mario Stampanoni;Iezzi, Ennio;Buttari, Fabio
通讯作者：
Buttari, Fabio
影响因子：
9.9
作者：
Lublin FD;Reingold SC;Cohen JA;Cutter GR;Sørensen PS;Thompson AJ;Wolinsky JS;Balcer LJ;Banwell B;Barkhof F;Bebo B Jr;Calabresi PA;Clanet M;Comi G;Fox RJ;Freedman MS;Goodman AD;Inglese M;Kappos L;Kieseier BC;Lincoln JA;Lubetzki C;Miller AE;Montalban X;O'Connor PW;Petkau J;Pozzilli C;Rudick RA;Sormani MP;Stüve O;Waubant E;Polman CH
通讯作者：
Polman CH
DOI：
10.1073/pnas.95.10.5768
发表时间：
1998-05-12
影响因子：
11.1
作者：
Shields, DC;Tyor, WR;Banik, NL
通讯作者：
Banik, NL