Dissecting gene expression at the blood-brain barrier.
Dissecting gene expression at the blood-brain barrier.
复制标题
DOI：
10.3389/fnins.2014.00355
复制
发表时间：
2014
影响因子：
4.3
通讯作者：
Watts RJ
中科院分区：
文献类型：
--
作者：
Huntley MA;Bien-Ly N;Daneman R;Watts RJ
血脑屏障全基因组表达数据的可用性是一种非常宝贵的资源，它最近使人们能够发现参与血脑屏障发育和维持的几个基因和途径，特别是在啮齿动物模型中。已发表数据集的广泛分布为血脑屏障的分子解剖提供了一个可行的起点，并将进一步指导发现血脑屏障形成和功能的新机制。脑内皮细胞是形成关键屏障的关键细胞，在纯化脑内皮细胞方面的技术进步使得与其他中枢神经系统细胞类型的基因表达比较具有更大的特异性，并且可以更系统地表征血脑屏障的分子组成。然而，我们对血脑屏障在衰老和疾病过程中如何变化的理解还不够充分。来自更广泛的实验范式和物种（包括无脊椎动物和灵长类动物）的血脑屏障数据集对于研究血脑屏障的功能和进化将是非常宝贵的。基因表达谱的新技术，如rna测序，现在允许更精细的转录组变化分辨率，包括同种异构体特异性和rna编辑。随着我们的领域继续利用更先进的表达谱来阐明血脑屏障，包括疾病和药物传递，我们将继续看到我们对屏障生物学分子介质的理解取得快速进展。我们预测，最近发表的数据集，结合即将到来的基因组和蛋白质组学血脑屏障数据集，将继续推动血脑屏障生物学的分子遗传革命。
The availability of genome-wide expression data for the blood-brain barrier is an invaluable resource that has recently enabled the discovery of several genes and pathways involved in the development and maintenance of the blood-brain barrier, particularly in rodent models. The broad distribution of published data sets represents a viable starting point for the molecular dissection of the blood-brain barrier and will further direct the discovery of novel mechanisms of blood-brain barrier formation and function. Technical advances in purifying brain endothelial cells, the key cell that forms the critical barrier, have allowed for greater specificity in gene expression comparisons with other central nervous system cell types, and more systematic characterizations of the molecular composition of the blood-brain barrier. Nevertheless, our understanding of how the blood-brain barrier changes during aging and disease is underrepresented. Blood-brain barrier data sets from a wider range of experimental paradigms and species, including invertebrates and primates, would be invaluable for investigating the function and evolution of the blood-brain barrier. Newer technologies in gene expression profiling, such as RNA-sequencing, now allow for finer resolution of transcriptomic changes, including isoform specificity and RNA-editing. As our field continues to utilize more advanced expression profiling in its ongoing efforts to elucidate the blood-brain barrier, including in disease and drug delivery, we will continue to see rapid advances in our understanding of the molecular mediators of barrier biology. We predict that the recently published data sets, combined with forthcoming genomic and proteomic blood-brain barrier data sets, will continue to fuel the molecular genetic revolution of blood-brain barrier biology.
登录
查看更多内容
DOI：
10.1016/s1385-299x(02)00147-2
发表时间：
2002-06-01
期刊：
BRAIN RESEARCH PROTOCOLS
影响因子：
--
作者：
Ball, HJ;McParland, B;Hunt, NH
通讯作者：
Hunt, NH
影响因子：
5.7
作者：
Dorr, A.;Sled, J. G.;Kabani, N.
通讯作者：
Kabani, N.
影响因子：
--
作者：
BETZ, AL;CSEJTEY, J;GOLDSTEIN, GW
通讯作者：
GOLDSTEIN, GW
影响因子：
6.4
作者：
Eadon, Michael T.;Jacob, Alexander;Alexander, Jessy J.
通讯作者：
Alexander, Jessy J.
影响因子：
3.7
作者：
Daneman R;Zhou L;Agalliu D;Cahoy JD;Kaushal A;Barres BA
通讯作者：
Barres BA