BMP-2 can promote the osteogenic differentiation of human endometrial stem cells
BMP-2 can promote the osteogenic differentiation of human endometrial stem cells
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BMP-2可促进人子宫内膜干细胞成骨分化
DOI：
10.5372/1905-7415.0704.258
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发表时间：
2014
影响因子：
0.6
通讯作者：
Ali Akar Alizadeh
中科院分区：
文献类型：
--
作者：
J. Ai;E. Azizi;Azam Shamsian;Akram Eslami;A. Khoshzaban;S. Ebrahimi‐Barough;Armin Ai;Ali Akar Alizadeh
摘要背景：人骨髓源性干细胞（hEnSC）是骨组织工程领域中最有价值的候选细胞。然而，骨形态发生蛋白-2（BMP-2）作为骨诱导生长因子的作用尚未明确确定。目的：探讨骨诱导生长因子BMP-2对人成骨干细胞成骨分化的促进作用。方法：子宫内膜活检取自因不孕症就诊的健康妇女。在胶原酶中消化组织后，分离的子宫内膜细胞在补充有10%FBS的Dulbecco改良Eagle培养基（DMEM）中扩增。基于子宫内膜（CD 90、CD 105）、内皮（CD 31）和造血（CD 34、CD 133）干细胞标志物的表达对增殖细胞进行表征。在BMP-2存在或不存在下，在含有补充有10%FBS、10 nM地塞米松、50 μg/ml抗坏血酸和10 mM β-甘油磷酸盐的DMEM的成骨培养基中分化细胞21天。进行茜素红染色以验证基质矿化。免疫细胞化学染色检测OCT-4、CD 133、骨桥蛋白和骨钙素的表达。半定量PCR检测成骨细胞骨桥蛋白、骨粘连蛋白和碱性磷酸酶的表达。结果：扩张的EnSC呈纺锤形。Oct-4、CD 90和CD 105表达阳性，而内皮和造血标志物表达阴性。在第21天，通过茜素红证实两组中的基质矿化。虽然骨桥蛋白和骨钙素的表达在两组中检测到免疫染色，骨钙素的表达更强烈的BMP-2的存在。ALP，骨连接素和骨桥蛋白转录本在所有组中表达，然而，在BMP-2的存在下，ALP和骨桥蛋白的表达上调。结论：BMP-2作为一种骨诱导生长因子，可促进体外培养的EnSCs向成骨细胞分化。
Abstract Background: Human endometrial-derived stem cells (hEnSCs) as multipotent accessible source of cells are known as useful cell candidates in the field of bone tissue engineering. However, the effect of bone morphogenic protein-2 (BMP-2) as an osteoinductive growth factor has not been clearly ascertained. Objective: To evaluate the effect of the remarkable osteoinductive growth factor BMP-2, on promotion of osteogenic differentiation in hEnSCs. Methods: Endometrial biopsies were obtained from healthy women referred to the hospital for infertility treatment. After tissue digestion in collagenase, the isolated endometrial cells were expanded in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% FBS. The propagated cells were characterized based on the expression of endometrial (CD90, CD105), endothelial (CD31), and hematopoietic (CD34, CD133) stem cell markers. Cells were differentiated in osteogenic medium containing DMEM supplemented with 10% FBS, 10 nM dexamethasone, 50 μg/ml Ascorbic acid, and 10 mM β-glycerophosphate in the presence or absence of BMP-2 for 21 days. Alizarin red staining was performed to verify the matrix mineralization. Immunocytochemical staining was conducted to detect the expression of OCT-4, CD133, and osteopontin as well as osteocalcin. The expression of osteoblast transcripts, including osteopontin, osteonectin, and alkaline phosphatase (ALP) were analyzed by semi quantitative PCR. Results: The expanded EnSCs were spindle shaped. They were positive for the expression of Oct-4, CD90, and CD105, while they were negative for endothelial and hematopoietic markers. The matrix mineralization was confirmed by Alizarin red in both groups at day 21. Although the expression of osteopontin and osteocalcin was detected in both groups by immunological staining, the expression of osteocalcin was more intense in the presence of BMP-2. ALP, Osteonectin and osteopontin transcripts were expressed in all groups; however, the expression of ALP and osteopontin was upregulated in the presence of BMP-2. Conclusion: BMP-2 as an osteoinductive growth factor, could promote the osteogenic differentiation of EnSCs in vitro.
影响因子：
4.1
作者：
Gundberg, CM;Looker, AC;Calvo, MS
通讯作者：
Calvo, MS
影响因子：
4.1
作者：
Edgar, Cory M.;Chakravarthy, Vinay;Einhorn, Thomas A.
通讯作者：
Einhorn, Thomas A.
影响因子：
3.6
作者：
PADYKULA, HA;COLES, LG;KAISERMANABRAMOF, IR
通讯作者：
KAISERMANABRAMOF, IR