Yersinia entry into host cells requires Rab5-dependent dephosphorylation of PI(4,5)P₂ and membrane scission.
Yersinia entry into host cells requires Rab5-dependent dephosphorylation of PI(4,5)P₂ and membrane scission.
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DOI：
10.1016/j.chom.2012.01.010
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发表时间：
2012-02-16
影响因子：
30.3
通讯作者：
Grinstein S
中科院分区：
文献类型：
--
作者：
Sarantis H;Balkin DM;De Camilli P;Isberg RR;Brumell JH;Grinstein S
一些细菌通过触发类似吞噬作用的过程侵入宿主细胞。我们分析了假结核耶尔森菌入侵液泡形成的机制。在与宿主细胞上的整合素结合后，耶尔森菌驻留在富含PI(4,5) p2的膜结合室中，称为“前孔”，细胞外抗体无法进入，但仍与质膜相连。前孔的断裂形成独立的、密封的隔室与PI(4,5)P2水解相吻合，并且需要PI(4,5)P2水解。在断裂时，肌醇5-磷酸酶ocl和Inpp5b被招募来预防孔洞。这种招募和随后的PI(4,5)P2水解需要GTPase Rab5与前泡的关联。磷脂酰肌醇3-激酶活性是rab5阳性囊泡与前泡融合和从质膜上分裂新生液泡的必需条件。因此，耶尔森氏菌的入侵涉及一个多步骤的过程，在这个过程中，细菌形成一个保护性的前孔，然后招募宿主因子诱导膜裂变，使细菌能够入侵细胞。
Some bacteria invade host cells by triggering a process akin to phagocytosis. We analyzed the mechanisms underlying invasion vacuole formation by the bacterium Yersinia pseudotuberculosis. After engaging integrins on host cells, Yersinia resided in PI(4,5)P2-rich, membrane-bound compartments called “prevacuoles” that were inaccessible to extracellular antibodies but remained connected to the plasma membrane. The scission of prevacuoles to form separate, sealed compartments coincided with and required PI(4,5)P2 hydrolysis. At the time of scission, the inositol 5-phosphatases OCRL and Inpp5b were recruited to prevacuoles. This recruitment and subsequent PI(4,5)P2 hydrolysis required the association of the GTPase Rab5 with prevacuoles. Phosphatidylinositol 3-kinase activity was required for fusion of Rab5-positive vesicles with prevacuoles and scission of nascent vacuoles from the plasma membrane. Thus, Yersinia invasion involves a multistep process in which the bacteria form a protective prevacuole and then recruit host factors to induce membrane fission, allowing the bacteria to invade the cell.
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影响因子：
11.8
作者：
Chang-Ileto, Belle;Frere, Samuel G.;Chan, Robin B.;Voronov, Sergey V.;Roux, Aurelian;Di Paolo, Gilbert
通讯作者：
Di Paolo, Gilbert
影响因子：
64.5
作者：
ISBERG, RR;LEONG, JM
通讯作者：
LEONG, JM
影响因子：
3.1
作者：
ROSENSHINE, I;DURONIO, V;FINLAY, BB
通讯作者：
FINLAY, BB
影响因子：
20.3
作者：
Herre, J;Marshall, ASJ;Brown, GD
通讯作者：
Brown, GD
影响因子：
16.2
作者：
Mani, Meera;Lee, Sang Yoon;Ryan, Timothy A.
通讯作者：
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