Acute modulation of synaptic plasticity of pyramidal neurons by activin in adult hippocampus.
Acute modulation of synaptic plasticity of pyramidal neurons by activin in adult hippocampus.
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DOI：
10.3389/fncir.2014.00056
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发表时间：
2014
影响因子：
3.5
通讯作者：
Kawato S
中科院分区：
文献类型：
--
作者：
Hasegawa Y;Mukai H;Asashima M;Hojo Y;Ikeda M;Komatsuzaki Y;Ooishi Y;Kawato S
激活素A被认为是在海马的急性兴奋性毒性损伤（在病理状态下）时产生的神经保护因子。我们试图揭示激活素在生理条件下（健康状态下）在成年男性海马中作为神经调质的作用，这在很大程度上仍是未知的。我们发现海马神经元中激活素的内源性/基础表达。树突棘（=突触后）中激活素受体的定位通过免疫电镜证实。用激活素A（10 ng/mL，0.4 nM）孵育海马急性切片2 h，改变了CA 1锥体神经元棘的密度和形态。激活素处理后，总棘密度增加了1.2倍。激活素选择性地增加大头棘的密度，而不影响中头和小头棘。阻断Erk/MAPK、PKA或PKC通过降低大头棘的密度来阻止激活素诱导的棘发生，而不依赖于Smad诱导的基因转录，这通常需要几个小时以上。急性脑片与激活素孵育2小时诱导了弱θ爆发刺激后的中度早期长时程增强（中度LTP）。卵泡抑素、MAPK抑制剂（PD98059）和NMDA受体NR2B亚单位抑制剂（Ro25 - 6981）可阻断这种中等程度的LTP诱导。应该注意的是，单独的弱θ爆发刺激不能诱导中度LTP。这些结果表明，MAPK诱导的NMDA受体（包括NR2B）的磷酸化可能在激活素诱导的中度LTP中发挥重要作用。两者合计，目前的结果揭示了有趣的生理作用的内源性激活素作为一种快速突触调制器在成年海马。
Activin A is known as a neuroprotective factor produced upon acute excitotoxic injury of the hippocampus (in pathological states). We attempt to reveal the role of activin as a neuromodulator in the adult male hippocampus under physiological conditions (in healthy states), which remains largely unknown. We showed endogenous/basal expression of activin in the hippocampal neurons. Localization of activin receptors in dendritic spines (=postsynapses) was demonstrated by immunoelectron microscopy. The incubation of hippocampal acute slices with activin A (10 ng/mL, 0.4 nM) for 2 h altered the density and morphology of spines in CA1 pyramidal neurons. The total spine density increased by 1.2-fold upon activin treatments. Activin selectively increased the density of large-head spines, without affecting middle-head and small-head spines. Blocking Erk/MAPK, PKA, or PKC prevented the activin-induced spinogenesis by reducing the density of large-head spines, independent of Smad-induced gene transcription which usually takes more than several hours. Incubation of acute slices with activin for 2 h induced the moderate early long-term potentiation (moderate LTP) upon weak theta burst stimuli. This moderate LTP induction was blocked by follistatin, MAPK inhibitor (PD98059) and inhibitor of NR2B subunit of NMDA receptors (Ro25-6981). It should be noted that the weak theta burst stimuli alone cannot induce moderate LTP. These results suggest that MAPK-induced phosphorylation of NMDA receptors (including NR2B) may play an important role for activin-induced moderate LTP. Taken together, the current results reveal interesting physiological roles of endogenous activin as a rapid synaptic modulator in the adult hippocampus.
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DOI：
10.1074/jbc.m110.121087
发表时间：
2010-10-22
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Arana-Argáez VE;Delgado-Rizo V;Pizano-Martínez OE;Martínez-Garcia EA;Martín-Márquez BT;Muñoz-Gómez A;Petri MH;Armendáriz-Borunda J;Espinosa-Ramírez G;Zúñiga-Tamayo DA;Herrera-Esparza R;Vázquez-Del Mercado M
通讯作者：
Vázquez-Del Mercado M
影响因子：
3.3
作者：
Hughes, PE;Alexi, T;Gluckman, PD
通讯作者：
Gluckman, PD
DOI：
10.1016/0006-291x(90)91544-3
发表时间：
1990-11-15
影响因子：
3.1
作者：
HERBERT, JM;AUGEREAU, JM;MAFFRAND, JP
通讯作者：
MAFFRAND, JP
DOI：
10.1073/pnas.92.17.7686
发表时间：
1995-08-15
影响因子：
11.1
作者：
DUDLEY, DT;PANG, L;SALTIEL, AR
通讯作者：
SALTIEL, AR
DOI：
10.1254/jjp.75.87
发表时间：
1997-09-01
期刊：
JAPANESE JOURNAL OF PHARMACOLOGY
影响因子：
--
作者：
Ikegaya, Y;Saito, H;Nishiyama, N
通讯作者：
Nishiyama, N