Amitotic neuroblastoma cells used for neural implants in monkeys.
Amitotic neuroblastoma cells used for neural implants in monkeys.
复制标题
用于猴子神经植入的无丝分裂神经母细胞瘤细胞。
作者：
D. Gash;M. F. Notter;S. Okawara;A. L. Kraus;R. Joynt
培养的神经母细胞瘤细胞作为供体组织的潜在效用中性植入到哺乳动物的大脑已被检查。来自人神经母细胞瘤细胞系IMR-32的细胞用[3 H]胸苷标记并化学地呈现无丝分裂。将这些分化的IMR-32细胞移植到5只成年非洲绿色猴的腰肌中，并在移植后评估移植物存活长达270天。在4只动物中鉴定了放射自显影标记的移植细胞。移植细胞的过程可以跟踪到宿主大脑中长达150微米的距离。没有发现移植物肿瘤生长的证据，因此，移植的神经母细胞瘤细胞可以在灵长类动物的大脑中存活很长时间，并可能作为神经植入物的供体组织的实际来源。
The potential utility of cultured neuroblastoma cells as donor tissue for neutral implants into the mammalian brain has been examined. Cells from a human neuroblastoma cell line, IMR-32, were labeled with [3H]thymidine and chemically rendered amitotic. These differentiated IMR-32 cells were grafted into the hippocampi of five adult African Green monkeys, and graft survival was evaluated for up to 270 days after transplantation. Autoradiographically labeled grafted cells were identified in four animals. Processes from grafted cells could be followed for distances of up to 150 micrometers into the host brain. No evidence for neoplastic growth of the transplant was found. Thus, grafted neuroblastoma cells can survive for prolonged periods in the primate brain and may serve as a practical source of donor tissue for neural implants.
移植的加压素神经元的功能发育。
DOI：
10.1126/science.7434031
发表时间：
1980
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gash,D;SladekJr,JR;Sladek,CD
通讯作者：
Sladek,CD