Molecular profile of cochlear immunity in the resident cells of the organ of Corti.
Molecular profile of cochlear immunity in the resident cells of the organ of Corti.
复制标题
柯蒂氏器常驻细胞中耳蜗免疫的分子特征。
DOI：
10.1186/s12974-014-0173-8
复制
发表时间：
2014-10-14
影响因子：
9.3
通讯作者：
Hu BH
中科院分区：
文献类型：
--
作者：
Cai Q;Vethanayagam RR;Yang S;Bard J;Jamison J;Cartwright D;Dong Y;Hu BH
耳蜗是听觉的感觉器官。在耳蜗里，Corti的器官容纳着对病理性侮辱敏感的感觉细胞。虽然科尔蒂的器官缺乏免疫细胞，但它确实具有免疫活动的能力。我们假设Corti器官中的驻留细胞负责应激诱导的Corti器官的免疫反应。这项研究描绘了Corti器官中免疫系统的分子组成，并检测了非免疫上皮细胞对声过度刺激的免疫反应。使用高通量RNA测序和qRT-PCR阵列，我们在耳蜗感觉上皮和Corti器官中鉴定了免疫和炎症相关基因。利用生物信息学分析，我们对表达的免疫基因进行了编目。然后，我们研究了这些基因对声过度刺激的反应，并确定了免疫基因表达的变化与感觉细胞损伤的关系。RNA测序分析表明，免疫相关基因在耳蜗感觉上皮细胞中表达旺盛。QRT-PCR阵列分析证实，这些基因中的许多在Corti器官的驻留细胞中结构性表达。生物信息学分析表明，表达的基因与Toll样受体信号通路有关。我们证明Toll样受体信号基因的表达主要来自Corti细胞器官中的支持细胞。重要的是，我们的数据表明，这些Toll样受体通路基因能够对声创伤做出反应，并且它们的表达变化与感觉细胞损伤有关。耳蜗器中的耳蜗驻留细胞具有免疫能力，参与耳蜗声过度刺激的免疫反应。
The cochlea is the sensory organ of hearing. In the cochlea, the organ of Corti houses sensory cells that are susceptible to pathological insults. While the organ of Corti lacks immune cells, it does have the capacity for immune activity. We hypothesized that resident cells in the organ of Corti were responsible for the stress-induced immune response of the organ of Corti. This study profiled the molecular composition of the immune system in the organ of Corti and examined the immune response of non-immune epithelial cells to acoustic overstimulation. Using high-throughput RNA-sequencing and qRT-PCR arrays, we identified immune- and inflammation-related genes in both the cochlear sensory epithelium and the organ of Corti. Using bioinformatics analyses, we cataloged the immune genes expressed. We then examined the response of these genes to acoustic overstimulation and determined how changes in immune gene expression were related to sensory cell damage. The RNA-sequencing analysis reveals robust expression of immune-related genes in the cochlear sensory epithelium. The qRT-PCR array analysis confirms that many of these genes are constitutively expressed in the resident cells of the organ of Corti. Bioinformatics analyses reveal that the genes expressed are linked to the Toll-like receptor signaling pathway. We demonstrate that expression of Toll-like receptor signaling genes is predominantly from the supporting cells in the organ of Corti cells. Importantly, our data demonstrate that these Toll-like receptor pathway genes are able to respond to acoustic trauma and that their expression changes are associated with sensory cell damage. The cochlear resident cells in the organ of Corti have immune capacity and participate in the cochlear immune response to acoustic overstimulation.
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影响因子：
6.1
作者：
Cai, Qunfeng;Patel, Minal;Coling, Donald;Hu, Bo Hua
通讯作者：
Hu, Bo Hua
影响因子：
2.8
作者：
FLOCK, A;BRETSCHER, A;WEBER, K
通讯作者：
WEBER, K
DOI：
10.1023/b:neur.0000005602.18713.02
发表时间：
2003-02-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
Jensen-Smith, HC;Eley, J;Hallworth, R
通讯作者：
Hallworth, R
影响因子：
3
作者：
Cai, Qunfeng;Wang, Bo;Patel, Minal;Yang, Shi Ming;Hu, Bo Hua
通讯作者：
Hu, Bo Hua
影响因子：
2.8
作者：
Hu, BH;Henderson, D;Nicotera, TM
通讯作者：
Nicotera, TM