PG1659 functions as anti-sigma factor to extracytoplasmic function sigma factor RpoE in Porphyromonas gingivalis W83.
PG1659 functions as anti-sigma factor to extracytoplasmic function sigma factor RpoE in Porphyromonas gingivalis W83.
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PG1659对牙龈卟啉单胞菌W83的胞质外功能sigma因子RpoE具有抗sigma因子的作用。
DOI：
10.1111/omi.12329
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发表时间：
2021-03
影响因子：
3.7
通讯作者：
Fletcher HM
中科院分区：
文献类型：
--
作者：
Dou Y;Rutanhira H;Schormann N;Deivanayagam C;Fletcher HM
抗σ因子在调节σ因子的表达以响应环境胁迫信号中起关键作用。PG 1659与上游基因PG 1660（rpoE）共转录，其编码在牙龈卟啉单胞菌的氧化应激抗性和毒力调节网络中起重要作用的σ因子。在本研究中评估了被注释为假设基因的PG 1659。PG 1659由130个氨基酸组成，被预测为具有单个钙（Ca 2+）结合位点的跨膜蛋白。在牙龈卟啉单胞菌FLL 358（Δ PG 1659：：ermF）中，与野生型W83菌株相比，rpoE基因高度上调。在PG 1659缺陷的同基因突变体中，RpoE诱导的基因也上调。蛋白质-蛋白质下拉和细菌双杂交试验均显示PG 1659蛋白可以与RpoE相互作用/结合。PG 1659的N-末端结构域代表蛋白质的胞质片段，对于与RpoE的相互作用至关重要。在PG 1659的存在下，RpoE σ因子的转录起始被抑制。综上所述，我们的数据表明，PG 1659是一种抗sigma因子，在牙龈卟啉单胞菌中sigma因子RpoE的调节中发挥重要的调节作用。
Anti-sigma factors play a critical role in regulating the expression of sigma factors in response to environmental stress signals. PG1659 is cotranscribed with an upstream gene PG1660 (rpoE), which encodes for a sigma factor that plays an important role in oxidative stress resistance and the virulence regulatory network of P. gingivalis. PG1659, which is annotated as a hypothetical gene, is evaluated in this study. PG1659, composed of 130 amino acids, is predicted to be transmembrane protein with a single calcium (Ca2+) binding site. In P. gingivalis FLL358 (ΔPG1659::ermF), the rpoE gene was highly upregulated compared to the wild-type W83 strain. RpoE induced genes were also upregulated in the PG1659-defective isogenic mutant. Both protein-protein pull-down and bacterial two-hybrid assays revealed that the PG1659 protein could interact with/bind RpoE. The N-terminal domain of PG1659, representing the cytoplasmic fragment of the protein, is critical for interaction with RpoE. In the presence of PG1659, the initiation of transcription by the RpoE sigma factor was inhibited. Taken together, our data suggest that PG1659 is an anti-sigma factor which plays an important regulatory role in the modulation of the sigma factor RpoE in P. gingivalis.
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DOI：
10.1073/pnas.0611567104
发表时间：
2007-03-06
影响因子：
11.1
作者：
Cezairliyan, Brent O.;Sauer, Robert T.
通讯作者：
Sauer, Robert T.
影响因子：
3.1
作者：
Henry LG;McKenzie RM;Robles A;Fletcher HM
通讯作者：
Fletcher HM
影响因子：
4.5
作者：
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通讯作者：
Holmstrup, Palle
影响因子：
4.8
作者：
Hardwick, Steven W.;Pane-Farre, Jan;Lewis, Richard J.
通讯作者：
Lewis, Richard J.
影响因子：
3.2
作者：
Delumeau, O;Lewis, RJ;Yudkin, MD
通讯作者：
Yudkin, MD