Diagnostic accuracy of a rapid influenza test for pandemic influenza A H1N1.
Diagnostic accuracy of a rapid influenza test for pandemic influenza A H1N1.
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DOI：
10.1371/journal.pone.0010364
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发表时间：
2010-04-28
期刊：
影响因子：
3.7
通讯作者：
Harris E
中科院分区：
文献类型：
--
作者：
Gordon A;Videa E;Saborío S;López R;Kuan G;Balmaseda A;Harris E
随着当前甲型H1N1流感大流行（H1N1 pdm），临床医生能够快速准确地识别流感病例是极其重要的。为了研究QuickVue Influenza A+B快速检测试剂盒的性能，我们在2至14岁的巴布亚新几内亚儿童中进行了一项前瞻性研究，比较了QuickVue Influenza A+B检测试剂盒与实时逆转录酶-聚合酶链反应（RT-PCR）对甲型H1N1 pdm流感的诊断准确性。与实时RT-PCR相比，快速检测的灵敏度和特异性分别为64.1%（95% CI 53.5，73.9）和98.3%（95.0，99.6）。两种检验之间的一致性为86.4%（95% CI 81.7，90.3），kappa计算为0.67（95% CI 0.56，0.76）。快速检测的性能因就诊日而异，对于症状发作当天就诊的儿童样本的灵敏度为41.7%（95%CI 22.1，63.4），对于症状发作后1天或1天以上就诊的儿童样本的灵敏度为72.1%（95%CI 59.9，82.3）。我们发现，快速测试执行具有中等灵敏度和高特异性。测试性能因发作日期而异，症状发作当天灵敏度较低。
With the current influenza A H1N1 pandemic (H1N1pdm), it is extremely important that clinicians can quickly and accurately identify influenza cases. To investigate the performance of the QuickVue Influenza A+B rapid test, we conducted a prospective study of the diagnostic accuracy of the QuickVue Influenza A+B test compared to real-time reverse transcriptase-polymerase chain reaction (RT-PCR) for influenza A H1N1pdm in Nicaraguan children aged 2 to 14 years. Rapid test sensitivity and specificity compared to real-time RT-PCR were 64.1% (95% CI 53.5, 73.9) and 98.3% (95.0, 99.6), respectively. Agreement between the two tests was 86.4% (95% CI 81.7, 90.3), and kappa was calculated to be 0.67 (95% CI 0.56, 0.76). Performance of the rapid test varied by day of presentation, with a sensitivity of 41.7% (95% CI 22.1, 63.4) for samples from children presenting on the day of symptom onset and a sensitivity of 72.1% (95% CI 59.9, 82.3) for samples from children presenting one or more days post-symptom onset. We found that the rapid test performed with moderate sensitivity and high specificity. Test performance varied by day of onset, with lower sensitivity on the day of symptom onset.
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影响因子：
3.7
作者：
Gordon, Aubree;Videa, Elsa;Harris, Eva
通讯作者：
Harris, Eva
影响因子：
11.8
作者：
Uyeki, Timothy M.;Prasad, Ramakrishna;Monto, Arnold S.
通讯作者：
Monto, Arnold S.
DOI：
10.1016/j.jcv.2009.06.005
发表时间：
2009-07
期刊：
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
影响因子：
--
作者：
Ginocchio CC;Zhang F;Manji R;Arora S;Bornfreund M;Falk L;Lotlikar M;Kowerska M;Becker G;Korologos D;de Geronimo M;Crawford JM
通讯作者：
Crawford JM
影响因子：
8
作者：
Grijalva, Carlos G.;Poehling, Katherine A.;Griffin, Marie R.
通讯作者：
Griffin, Marie R.
影响因子：
--
作者：
Poehling, KA;Zhu, YW;Edwards, K
通讯作者：
Edwards, K