Anti- Versus Pro-Inflammatory Metabololipidome Upon Cupping Treatment
Anti- Versus Pro-Inflammatory Metabololipidome Upon Cupping Treatment
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拔罐治疗后的抗炎代谢脂组与促炎代谢脂组
DOI：
10.1159/000487563
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发表时间：
2018-02
影响因子：
--
通讯作者：
Li Yongsheng
中科院分区：
文献类型：
--
作者：
Zhang Qi;Wang Xiang;Yan Guifang;Lei Juan;Zhou Yu;Wu Lei;Wang Ting;Zhang Xiao;Ye Duyun;Li Yongsheng
背景/目的：本研究旨在探讨拔火罐对小鼠代谢脂组的影响。方法：建立模拟人体拔罐治疗的裸鼠模型，对裸鼠背部皮肤进行4组拔罐，拔罐时间15分钟。采用UPLC-MS/ MS法测定拔罐前后小鼠皮肤和血液中PUFA代谢组。在巨噬细胞中给予显著改变的脂质，通过ELISA评估促炎细胞因子IL-6和TNF-α的产生。结果：拔罐后皮肤或血浆中PGE1、5、6-EET、14、15-EET、10S、17S-DiHDoHE、17R-RvD1、RvD5、14S-HDoHE等抗炎脂质明显升高，12-HETE、TXB2等促炎脂质明显降低。拔火罐可逆转lps刺激的小鼠腹膜渗出液中IL-6和TNF-α的表达。此外，5,6- eet、PGE1降低巨噬细胞TNF-α水平，而5,6- eet、5,6- dhet下调巨噬细胞IL-6的产生。重要的是，14,15- eet和14S-HDoHE抑制脂多糖（LPS）诱导的IL-6和TNF-α。17-RvD1、RvD5和PGE1显著降低lps诱导的TNF-α，而TXB2和12-HETE进一步上调lps增强的巨噬细胞IL-6和TNF-α的表达。结论：我们的研究结果揭示了抗炎和促炎代谢脂组的特性，并提示了拔罐治疗的潜在治疗机制。
Background/Aims: This study aimed to explore the metabololipidome in mice upon cupping treatment. Methods: A nude mouse model mimicking the cupping treatment in humans was established by administrating four cupping sets on the back skin for 15 minutes. UPLC-MS/ MS was performed to determine the PUFA metabolome in mice skin and blood before and after cupping treatment. The significantly changed lipids were administered in macrophages to assess the production of pro-inflammatory cytokines IL-6 and TNF-α by ELISA. Results: The anti-inflammatory lipids, e.g. PGE1, 5,6-EET, 14,15-EET, 10S,17S-DiHDoHE, 17R-RvD1, RvD5 and 14S-HDoHE were significantly increased while pro-inflammatory lipids, e.g. 12-HETE and TXB2 were deceased in the skin or plasma post cupping treatment. Cupping treatment reversed the LPS-stimulated IL-6 and TNF-α expression in mouse peritoneal exudates. Moreover, 5,6-EET, PGE1 decreased the level of TNF-α, while 5,6-EET, 5,6-DHET downregulated IL-6 production in macrophages. Importantly, 14,15-EET and 14S-HDoHE inhibited both IL-6 and TNF-α induced by lipopolysaccharide (LPS). 17-RvD1, RvD5 and PGE1 significantly reduced the LPS-initiated TNF-α, while TXB2 and 12-HETE further upregulated the LPS-enhanced IL-6 and TNF-α expression in macrophages. Conclusion: Our results reveal the identities of anti-inflammatory versus pro-inflammatory metabolipidome and suggest the potential therapeutic mechanism of cupping treatment.
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影响因子：
2.7
作者：
van Erk MJ;Wopereis S;Rubingh C;van Vliet T;Verheij E;Cnubben NH;Pedersen TL;Newman JW;Smilde AK;van der Greef J;Hendriks HF;van Ommen B
通讯作者：
van Ommen B
影响因子：
0.7
作者：
Pei-chang Xu;S. Cui;Derrik Aw Chen Wee;Sheng Xu;Lee Tat Leang
通讯作者：
Pei-chang Xu;S. Cui;Derrik Aw Chen Wee;Sheng Xu;Lee Tat Leang
影响因子：
64.8
作者：
Chiang, Nan;Fredman, Gabrielle;Backhed, Fredrik;Oh, Sungwhan F.;Vickery, Thad;Schmidt, Birgitta A.;Serhan, Charles N.
通讯作者：
Serhan, Charles N.
影响因子：
6.4
作者：
Srinivasan S;Morgan MT;Fiedler TL;Djukovic D;Hoffman NG;Raftery D;Marrazzo JM;Fredricks DN
通讯作者：
Fredricks DN
Omega-3 脂肪酸通过抑制 NLRP3 炎症小体激活来预防炎症和代谢紊乱。
影响因子：
32.4
作者：
Yan, Yiqing;Jiang, Wei;Zhou, Rongbin
通讯作者：
Zhou, Rongbin