Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study.
Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study.
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DOI：
10.1186/1755-8794-3-5
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发表时间：
2010-02-23
影响因子：
2.7
通讯作者：
van Ommen B
中科院分区：
文献类型：
--
作者：
van Erk MJ;Wopereis S;Rubingh C;van Vliet T;Verheij E;Cnubben NH;Pedersen TL;Newman JW;Smilde AK;van der Greef J;Hendriks HF;van Ommen B
肥胖受试者的慢性全身性低度炎症与健康并发症相关，包括心血管疾病、胰岛素抵抗和糖尿病。减少炎症反应可以降低这些风险。然而，现有的炎症状态标志物不足以描述轻度抗炎治疗的代谢反应的复杂性。为了解决这一局限性，我们使用了一种综合组学方法来表征在非甾体抗炎药双氯芬酸干预期间超重男性的炎症调节。测量的参数包括80种血浆蛋白、> 300种血浆代谢物（脂质、游离脂肪酸、氧化脂质和极性化合物）和一系列外周血单核细胞（PBMC）基因表达产物。这些措施被提交给多变量和相关性分析，并用于构建生物反应网络。鉴定了一组基因、蛋白质和代谢物，包括PGE2和TNF-α，其描述了一个双抗应答网络（PBMC中的68个基因，1个血浆蛋白和4个血浆代谢物）。炎症调节的新候选标志物包括PBMC膜联蛋白A1和半胱天冬酶8的表达，以及花生四烯酸代谢物5，6-DHET。在这项研究中，对广泛参数的综合分析允许开发响应炎症调节的标记物网络，从而提供对炎症复杂过程的洞察以及评估与肥胖相关的炎症状态变化的方法。该研究在www.example.com数据库中注册为NCT 00221052。
Chronic systemic low-grade inflammation in obese subjects is associated with health complications including cardiovascular diseases, insulin resistance and diabetes. Reducing inflammatory responses may reduce these risks. However, available markers of inflammatory status inadequately describe the complexity of metabolic responses to mild anti-inflammatory therapy. To address this limitation, we used an integrative omics approach to characterize modulation of inflammation in overweight men during an intervention with the non-steroidal anti-inflammatory drug diclofenac. Measured parameters included 80 plasma proteins, >300 plasma metabolites (lipids, free fatty acids, oxylipids and polar compounds) and an array of peripheral blood mononuclear cells (PBMC) gene expression products. These measures were submitted to multivariate and correlation analysis and were used for construction of biological response networks. A panel of genes, proteins and metabolites, including PGE2 and TNF-alpha, were identified that describe a diclofenac-response network (68 genes in PBMC, 1 plasma protein and 4 plasma metabolites). Novel candidate markers of inflammatory modulation included PBMC expression of annexin A1 and caspase 8, and the arachidonic acid metabolite 5,6-DHET. In this study the integrated analysis of a wide range of parameters allowed the development of a network of markers responding to inflammatory modulation, thereby providing insight into the complex process of inflammation and ways to assess changes in inflammatory status associated with obesity. The study is registered as NCT00221052 in clinicaltrials.gov database.
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影响因子：
4.8
作者：
Luria, Ayala;Weldon, Steven M.;Hammock, Bruce D.
通讯作者：
Hammock, Bruce D.
影响因子：
9.8
作者：
Browning, LM;Krebs, JD;Jebb, SA
通讯作者：
Jebb, SA
影响因子：
2.4
作者：
Barker, M;Rayens, W
通讯作者：
Rayens, W
影响因子：
4.9
作者：
Browning, LM;Jebb, SA;Krebs, JD
通讯作者：
Krebs, JD
DOI：
10.1152/ajpheart.00844.2002
发表时间：
2003-06-01
影响因子：
4.8
作者：
Stephenson, AH;Sprague, RS;Lonigro, AJ
通讯作者：
Lonigro, AJ