Antibiotics of Pseudomonas protegens FD6 are essential for biocontrol activity
Antibiotics of Pseudomonas protegens FD6 are essential for biocontrol activity
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保护假单胞菌 FD6 的抗生素对于生物防治活动至关重要
DOI：
10.1007/s13313-020-00696-7
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发表时间：
2020-04
影响因子：
1.4
通讯作者：
Chen Xiao J.
中科院分区：
文献类型：
--
作者：
Zhang Q. X.;Kong X. W.;Li S. Y.;Chen Xi J.;Chen Xiao J.
从油菜根际分离的假单胞菌Protegens（以前的荧光假单胞菌）FD 6是一种生物防治剂，其保护免受真菌病原体灰葡萄孢和果褐腐病菌的侵害。测定了6.7-Mb Protegens假单胞菌FD 6基因组的完整序列。菌株FD 6的基因组分析导致鉴定出12个推定的次级代谢物基因簇，包括抗生素2，4-二乙酰间苯三酚（2，4-DAPG）、绿脓杆菌素（PLT）和吡咯硝基菌素（PRN）。为了研究2，4-DAPG和PLT在保护类青霉FD 6生防活性中的作用，利用同源重组技术构建了两株保护类青霉FD 6突变株ΔphlC和ΔpltD。结果表明，ΔphlC突变体的拮抗作用与野生型FD 6相比无显著差异。然而，ΔpltD突变体不再表现出抗真菌活性。HPLC分析表明，ΔphlC突变体不能生物合成2，4-DAPG，而ΔphlC突变体中PLT产量比野生型FD 6显著增加67倍。在ΔpltD突变体中未检测到PLT的生物合成，而与菌株FD 6相比，2，4-DAPG的水平大大降低超过16倍。保护假单胞菌FD 6产生的主要拮抗活性对应于2，4-二乙酰基间苯三酚和苯丙酮酸。2，4-DAPG与PLT之间存在着相反的相互作用。
Pseudomonas protegens (formerly Pseudomonas fluorescens) FD6, which was isolated from the canola rhizosphere, is a biocontrol agent that protects against the fungal pathogens Botrytis cinerea and Monilinia fructicola. The complete sequence of the 6.7-Mb Pseudomonas protegens FD6 genome was determined. Genomic analysis of strain FD6 led to the identification of twelve putative gene clusters of secondary metabolites, including the antibiotics 2,4-diacetylphloroglucinol (2,4-DAPG), pyoluteorin (PLT) and pyrrolnitrin (PRN). To assess the role of 2,4-DAPG and PLT in the biocontrol activity of P. protegens FD6, two mutants of P. protegens FD6, including strains ΔphlC and ΔpltD, were constructed using homologous recombination technology. The results showed that no significant differences were observed in the antagonistic action of the ΔphlC mutant compared with wild-type FD6. However, the ΔpltD mutant no longer exhibited antifungal activity. HPLC analysis indicated that ΔphlC mutant could not biosynthesize 2,4-DAPG, whereas PLT production in the ΔphlC mutant was significantly increased by 67-fold compared with wild-type FD6. Biosynthesis of PLT in the ΔpltD mutant was not detected, whereas the level of 2,4-DAPG was vastly decreased over sixteenfold compared with strain FD6. The major antagonistic activity produced by P. protegens FD6 corresponded to 2,4-diacetylphloroglucinol and pyoluteorin. There is an inverse interaction between 2,4-DAPG and PLT.
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影响因子：
14.9
作者：
Witney AA;Waldron DE;Brooks LA;Tyler RH;Withers M;Stoker NG;Wren BW;Butcher PD;Hinds J
通讯作者：
Hinds J
假单胞菌属四种代表性植物根际促生细菌的比较基因组分析
影响因子：
4.4
作者：
Shen X;Hu H;Peng H;Wang W;Zhang X
通讯作者：
Zhang X
地狱1.1：RNA同源性搜索速度更快100倍。
DOI：
10.1093/bioinformatics/btt509
发表时间：
2013-11-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Nawrocki EP;Eddy SR
通讯作者：
Eddy SR
影响因子：
7.7
作者：
Qing Yan;B. Philmus;Jeff H. Chang;J. Loper
通讯作者：
Qing Yan;B. Philmus;Jeff H. Chang;J. Loper
影响因子：
3.2
作者：
D. Weller
通讯作者：
D. Weller