The ecotoxicological contaminant tris(4-chlorophenyl)methanol (TCPMOH) impacts embryonic development in zebrafish (Danio rerio).
The ecotoxicological contaminant tris(4-chlorophenyl)methanol (TCPMOH) impacts embryonic development in zebrafish (Danio rerio).
复制标题
生态毒理学污染物三（4-氯苯基）甲醇（TCPMOH）影响斑马鱼（Danio rerio）的胚胎发育。
DOI：
10.1016/j.aquatox.2021.105815
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发表时间：
2021-06
期刊：
影响因子：
--
通讯作者：
Sant KE
中科院分区：
文献类型：
--
作者：
Navarrete J;Wilson P;Allsing N;Gordon C;Margolis R;Schwartz AV;Cho C;Rogowski B;Topps J;George UZ;Sant KE
三（4-氯苯基）甲醇（TCPMOH）是一种病因不明的水污染物，但据信是滴滴涕生产的副产品。它在环境中具有高度持久性，并在海洋物种中生物积累。人类母乳中也测量了TCPMOH，这对发育中的婴儿构成了风险。然而，目前几乎没有毒性数据。在这项研究中，我们使用斑马鱼模型（Danio rerio）研究了TCPMOH发育暴露所造成的危害。斑马鱼（Danio rerio）胚胎在受精后24小时（hpf）开始暴露于0、0.1、0.5、1或5 μM TCPMOH。胚胎死亡率和形态畸形发生率随TCPMOH暴露呈浓度依赖性增加。RNA测序评估与急性（4小时）暴露于50nM TCPMOH相关的基因表达变化。发育期暴露于TCPMOH会降低ahr2以及代谢酶cyp1a1、cyp1b1、cyp1c1、cyp1c2和cyp2y3的表达（p<0.05）。这些结果与乙氧基间苯二酚- o -去乙基酶（EROD）测定的Cyp1a1诱导降低一致（p<0.05）。与异种代谢、脂质代谢以及转录和翻译调节相关的途径减少。参与DNA复制和修复、碳水化合物代谢和内分泌功能的途径上调。总之，本研究表明TCPMOH在高浓度下对斑马鱼胚胎具有急性毒性。
Tris(4-chlorophenyl)methanol (TCPMOH) is a water contaminant with unknown etiology, but is believed to be a byproduct of DDT manufacturing. It is highly persistent in the environment, and bioaccumulates in marine species. TCPMOH has also been measured in human breast milk, which poses a risk for developing infants. However, almost no toxicity data is currently available. In this study, we investigate the hazard posed by developmental TCPMOH exposures using the zebrafish model (Danio rerio). Zebrafish (Danio rerio) embryos were exposed to 0, 0.1, 0.5, 1, or 5 μM TCPMOH beginning at 24 hours post fertilization (hpf). Embryonic mortality and incidence of morphological deformities increased in a concentration-dependent manner with TCPMOH exposure. RNA sequencing assessed changes in gene expression associated with acute (4 hour) exposures to 50nM TCPMOH. Developmental exposure to TCPMOH decreased expression of ahr2, as well as metabolic enzymes cyp1a1, cyp1b1, cyp1c1, cyp1c2, and cyp2y3 (p<0.05). These findings were concordant with decreased Cyp1a1 induction measured by the ethoxyresorufin-O-deethylase (EROD) assay (p<0.05). Pathways associated with xenobiotic metabolism, lipid metabolism, and transcriptional and translational regulation were decreased. Pathways involved in DNA replication and repair, carbohydrate metabolism, and endocrine function were upregulated. Overall, this study demonstrates that TCPMOH is acutely toxic to zebrafish embryos at elevated concentrations.
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影响因子：
14.9
作者：
Kuleshov MV;Jones MR;Rouillard AD;Fernandez NF;Duan Q;Wang Z;Koplev S;Jenkins SL;Jagodnik KM;Lachmann A;McDermott MG;Monteiro CD;Gundersen GW;Ma'ayan A
通讯作者：
Ma'ayan A
影响因子：
10.4
作者：
通讯作者：
--
影响因子：
3.6
作者：
Andreasen, EA;Hahn, ME;Tanguay, RL
通讯作者：
Tanguay, RL
影响因子：
3.8
作者：
Hornug, MW;Spitsbergen, JM;Peterson, RE
通讯作者：
Peterson, RE
影响因子：
8.8
作者：
Foster, WG;Desaulniers, D;Chu, I
通讯作者：
Chu, I