Loop-Mediated Isothermal Amplification (LAMP): Emergence As an Alternative Technology for Herbal Medicine Identification.
Loop-Mediated Isothermal Amplification (LAMP): Emergence As an Alternative Technology for Herbal Medicine Identification.
复制标题
环介导等温扩增 (LAMP)：作为草药鉴定替代技术的出现
DOI：
10.3389/fpls.2016.01956
复制
发表时间：
2016
影响因子：
5.6
通讯作者：
Zhou XW
中科院分区：
文献类型：
--
作者：
Li JJ;Xiong C;Liu Y;Liang JS;Zhou XW
正确识别药用植物成分对于其安全使用和草药供应链的监管至关重要。环介导等温扩增（LAMP）是近年来发展起来的一种鉴定中草药的方法。这种新的分子生物学技术能够实现及时和准确的检测，特别是在缺乏支持聚合酶链反应设施的基础设施的环境中。使用这种方法的研究改变了我们对草药鉴定的范围和复杂性的看法。本文介绍了LAMP分析的基本原理和发展过程中的重要环节。然后，我们提出了一个关键性的审查，基于LAMP的方法在检测和鉴定药材及其加工产品的应用。我们还提供了一个实用的标准操作程序（SOP）的利用LAMP协议在草药认证，并考虑在未来的发展中草药鉴定的LAMP技术的前景和与其应用的挑战。
Correct identification of medicinal plant ingredients is essential for their safe use and for the regulation of herbal drug supply chain. Loop-mediated isothermal amplification (LAMP) is a recently developed approach to identify herbal medicine species. This novel molecular biology technique enables timely and accurate testing, especially in settings where infrastructures to support polymerase chain reaction facilities are lacking. Studies that used this method have altered our view on the extent and complexity of herbal medicine identification. In this review, we give an introduction into LAMP analysis, covers the basic principles and important aspects in the development of LAMP analysis method. Then we presented a critical review of the application of LAMP-based methods in detecting and identifying raw medicinal plant materials and their processed products. We also provide a practical standard operating procedure (SOP) for the utilization of the LAMP protocol in herbal authentication, and consider the prospects of LAMP technology in the future developments of herbal medicine identification and the challenges associated with its application.
登录
查看更多内容
影响因子：
5.6
作者：
Chen X;Wang X;Jin N;Zhou Y;Huang S;Miao Q;Zhu Q;Xu J
通讯作者：
Xu J
影响因子：
8.8
作者：
Huang, Xin;Chen, Lili;Chen, Hongjun
通讯作者：
Chen, Hongjun
影响因子：
2
作者：
Bandyopadhyay, S.;Raychaudhuri, S. S.
通讯作者：
Raychaudhuri, S. S.
影响因子：
3.7
作者：
Duan YB;Ge CY;Zhang XK;Wang JX;Zhou MG
通讯作者：
Zhou MG
影响因子：
3.3
作者：
Fukuta, S;Mizukami, Y;Kanbe, M
通讯作者：
Kanbe, M