Inhibitory effects of edaravone in β-amyloid-induced neurotoxicity in rats.
Inhibitory effects of edaravone in β-amyloid-induced neurotoxicity in rats.
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DOI：
10.1155/2014/370368
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发表时间：
2014
影响因子：
--
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
He F;Cao YP;Che FY;Yang LH;Xiao SH;Liu J
淀粉样蛋白可以通过多种生物机制损害神经细胞，包括氧化应激、钙稳态的改变和促凋亡。依达拉奉是一种有效的自由基清除剂，具有抗氧化作用，已被证明对中风患者具有神经保护作用。本研究旨在通过研究 Aβ 1-40 诱导的海马 CA1 锥体神经元中的电压门控钙通道电流、学习和记忆行为测试、基底前脑中存活的胆碱能神经元的数量以及 AD 大鼠模型中海马中的乙酰胆碱水平，探讨 EDA 对 Aβ 诱导的 AD 大鼠模型的影响。结果表明，EDA 可抑制 Aβ 1-40 诱导的 I Ca 增加，且呈剂量依赖性。 EDA 治疗显着改善了 Aβ 1-40 诱导的学习和记忆性能。与非 EDA 处理的 Aβ 1-40 组相比，给予 EDA 后，基底前脑中的胆碱乙酰转移酶阳性细胞和海马中的乙酰胆碱含量增加。这些结果表明EDA可以抑制Aβ毒性的神经毒性作用。总的来说，这些发现表明 EDA 可能作为 AD 的潜在补充治疗策略。
Amyloid protein can damage nerve cells through a variety of biological mechanisms including oxidative stress, alterations in calcium homeostasis, and proapoptosis. Edaravone, a potent free radical scavenger possessing antioxidant effects, has been proved neuroprotective effect in stroke patients. The current study aimed to investigate the effects of EDA in an Aβ-induced rat model of AD, by studying Aβ 1–40-induced voltage-gated calcium channel currents in hippocampal CA1 pyramidal neurons, learning and memory behavioral tests, the number of surviving cholinergic neurons in the basal forebrain, and the acetylcholine level in the hippocampus in this rat model of AD. The results showed that the Aβ 1–40-induced increase of I Ca can be inhibited by EDA in a dose-dependent manner. Treatment with EDA significantly improved Aβ 1–40-induced learning and memory performance. Choline acetyltransferase positive cells in basal forebrain and acetylcholine content in the hippocampus were increased by the administration of EDA as compared with the non-EDA treated Aβ 1–40 group. These results demonstrate that EDA can inhibit the neurotoxic effect of Aβ toxicity. Collectively, these findings suggest that EDA may serve as a potential complemental treatment strategy for AD.
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影响因子：
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