Diuretic Activity of Compatible Triterpene Components of Alismatis rhizoma.
Diuretic Activity of Compatible Triterpene Components of Alismatis rhizoma.
复制标题
泽泻配伍三萜成分的利尿活性
DOI：
10.3390/molecules22091459
复制
发表时间：
2017-09-06
期刊：
影响因子：
--
通讯作者：
Wu SS
中科院分区：
文献类型：
--
作者：
Zhang X;Li XY;Lin N;Zhao WL;Huang XQ;Chen Y;Huang MQ;Xu W;Wu SS
泽泻（Alismatis rhizoma， AR）是泽泻科植物泽泻的干燥根茎，是一种中药。AR是许多处方的重要组成部分，在亚洲通常用作利尿剂。本研究旨在评价AR总三萜提取物（TTE）和三萜组分相容性（TCC，即alisol b23 -acetate、alisol B、alisol A - 24-acetate、alisol A和alisol c23 -acetate的混合物）对盐负荷大鼠的利尿作用。采用均匀设计优化AR的最佳利尿剂TCC。大鼠口服不同剂量（5、20、40 mg/kg）的TTE和TCC组（n1 ~ n8）。测定含盐大鼠尿液的排泄率、pH值和电解质排泄量。结果显示，与对照组相比，TTE剂量增加了尿量和电解质排泄。所有统一设计的TCC组也增加了尿排泄量。此外，我们还计算了最佳利尿剂TCC (alisol B 23-acetate: alisol B: alisol A 24-acetate: alisol A: alisol C 23-acetate 7.2:0.6:2.8:3.0:6.4)，并通过盐负荷大鼠进行验证。本研究表明，TTE通过增加Na+、K+和Cl−位移而具有显著的利尿作用。验证了alisol b23 -acetate: alisol B: alisol A - 24-acetate: alisol A: alisol c23 -acetate最适宜的TTC配合比具有利尿活性，三萜是AR利尿活性的物质基础。
Alismatis rhizoma (AR), the dried rhizoma of Alisma orientale Juzepzuk (Alismataceae), is a traditional Chinese medicine. AR is an important part of many prescriptions and is commonly used as a diuretic agent in Asia. This study aimed to evaluate the diuretic effects of total triterpene extract (TTE) and triterpene component compatibility (TCC, the mixture of alisol B 23-acetate, alisol B, alisol A 24-acetate, alisol A, and alisol C 23-acetate) of AR in saline-loaded rats. The optimal diuretic TCC of AR was optimized using a uniform design. Different doses (5, 20, and 40 mg/kg) of TTE and TCC groups (N1–N8) were orally administered to rats. Urinary excretion rate, pH, and electrolyte excretion were measured in the urine of saline-loaded rats. Results showed that TTE doses increased urine volume and electrolyte excretion compared with the control group. All uniformly designed groups of TCC also increased urine excretion. In addition, optimal diuretic TCC was calculated (alisol B 23-acetate: alisol B: alisol A 24-acetate: alisol A: alisol C 23-acetate 7.2:0.6:2.8:3.0:6.4) and further validated by saline-loaded rats. This study demonstrated that TTE presented a notable diuretic effect by increasing Na+, K+, and Cl− displacements. The most suitable TTC compatible proportion of alisol B 23-acetate: alisol B: alisol A 24-acetate: alisol A: alisol C 23-acetate for diuretic activity was validated, and triterpenes were the material basis for the diuretic activity of AR.
登录
查看更多内容
泽泻：重要中药的民族药理学、植物化学和药理学
影响因子：
5.7
作者：
Shu, Zhiheng;Pu, Jiang;Zheng, Chengjian
通讯作者：
Zheng, Chengjian
影响因子：
6.7
作者：
Hur, Jong Moon;Choi, Jong Won;Park, Jong Cheol
通讯作者：
Park, Jong Cheol
影响因子：
1.7
作者：
Li, Hong-Mei;Fan, Min;Zhao, Qin-Shi
通讯作者：
Zhao, Qin-Shi
Alisol A 24-Acetate 和 Alisol B 23-Acetate 诱导的自噬介导人肾近端管状细胞的凋亡和肾毒性
影响因子：
5.6
作者：
Wang C;Feng L;Ma L;Chen H;Tan X;Hou X;Song J;Cui L;Liu D;Chen J;Yang N;Wang J;Liu Y;Zhao B;Wang G;Zhou Y;Jia X
通讯作者：
Jia X
影响因子：
5.4
作者：
Yang, Ying;Zhang, Dong-Mei;Kong, Ling-Dong
通讯作者：
Kong, Ling-Dong